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MCL-1 anticorps (C-Term)

MCL1 Reactivité: Humain WB, IP Hôte: Souris Monoclonal 22-Mcl unconjugated
N° du produit ABIN967637
  • Antigène Voir toutes MCL-1 (MCL1) Anticorps
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    Épitope
    • 24
    • 22
    • 16
    • 11
    • 11
    • 9
    • 8
    • 8
    • 7
    • 7
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 148
    • 83
    • 71
    • 6
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 145
    • 8
    • 2
    • 1
    Souris
    Clonalité
    • 139
    • 19
    Monoclonal
    Conjugué
    • 73
    • 12
    • 12
    • 10
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MCL-1 est non-conjugé
    Application
    • 124
    • 66
    • 53
    • 45
    • 27
    • 26
    • 22
    • 22
    • 11
    • 9
    • 7
    • 5
    • 4
    Western Blotting (WB), Immunoprecipitation (IP)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Purified recombinant human Mcl-1 lacking the C-terminal hydrophobic domain
    Clone
    22-Mcl
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product MCL1 Anticorps primaire
  • Indications d'application
    The antibody is tested by Western blot on K-562 cell lysate (ATCC CCL-243). Other applications include immunoprecipitation (1-2 µg/ml).
    Commentaires

    Related Products: ABIN968586, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4° C.
  • Gross, McDonnell, Korsmeyer: "BCL-2 family members and the mitochondria in apoptosis." dans: Genes & development, Vol. 13, Issue 15, pp. 1899-911, (1999) (PubMed).

    Wang, Chao, Chen, Kuo, Yen, Yang-Yen: "The antiapoptotic gene mcl-1 is up-regulated by the phosphatidylinositol 3-kinase/Akt signaling pathway through a transcription factor complex containing CREB." dans: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6195-206, (1999) (PubMed).

    Adams, Cory: "The Bcl-2 protein family: arbiters of cell survival." dans: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, (1998) (PubMed).

    Yang, Kozopas, Craig: "The intracellular distribution and pattern of expression of Mcl-1 overlap with, but are not identical to, those of Bcl-2." dans: The Journal of cell biology, Vol. 128, Issue 6, pp. 1173-84, (1995) (PubMed).

    Kozopas, Yang, Buchan, Zhou, Craig: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, (1993) (PubMed).

    Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." dans: Cell, Vol. 74, Issue 4, pp. 609-19, (1993) (PubMed).

  • Antigène
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    Autre désignation
    Mcl-1 (MCL1 Produits)
    Synonymes
    anticorps BCL2L3, anticorps EAT, anticorps MCL1-ES, anticorps MCL1L, anticorps MCL1S, anticorps Mcl-1, anticorps TM, anticorps bcl2-L-3, anticorps mcl1/EAT, anticorps AW556805, anticorps mcl-1, anticorps fb72f02, anticorps wu:fb70a08, anticorps wu:fb72f02, anticorps zfMLP1, anticorps zfMcl-1a, anticorps zgc:109835, anticorps zgc:109763, anticorps E3, anticorps E3-2, anticorps RNE3, anticorps RNU107, anticorps SNORA63A, anticorps elF-4AII, anticorps MCL1, BCL2 family apoptosis regulator, anticorps myeloid cell leukemia sequence 1, anticorps MCL1, BCL2 family apoptosis regulator a, anticorps MCL1, BCL2 family apoptosis regulator b, anticorps small nucleolar RNA, H/ACA box 63, anticorps MCL1, anticorps Mcl1, anticorps mcl1a, anticorps mcl1b, anticorps SNORA63
    Sujet
    The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Mcl-1 is a member of the anti-apoptotic Bcl-2 group of proteins, including Bcl-x, Bcl-w, A1/Bfl-1, Boo/Diva, and Nr-13. Mcl-1 was cloned from the ML-1 human myeoblastic leukemia cell line. Mcl-1 localizes to the mitochrondria, like Bcl-2, but also associates with membranes via its C-terminal hydrophobic domain. Further studies have shown that Mcl-1 is upregulated in response to the GM-CSF and IL-3 cytokine signaling pathways and that this regulation of Mcl-1 is controlled at the level of transcription. Mcl-1 is detected at approximately 40 kDa in SDS/PAGE.
    Poids moléculaire
    40 kDa
    Pathways
    Signalisation MAPK
Support technique