RAS anticorps (AA 1-190)

N° du produit ABIN967686

Détail du produit anti-RAS anticorps

Antigène: RAS

Épitope: AA 1-190

Reactivité: Humain, Rat, Souris, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAS est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Poulet, Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Ras (Ha-ras) aa. 1-190

Clone: 18-Ras

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAS (ABIN967686)

Dhillon, Meikle, Yazici, Eulitz, Kolch: "Regulation of Raf-1 activation and signalling by dephosphorylation." dans: The EMBO journal, Vol. 21, Issue 1-2, pp. 64-71, (2002) (PubMed).

Garcia, de Gunzburg, Eychène, Gisselbrecht, Porteu: "Thrombopoietin-mediated sustained activation of extracellular signal-regulated kinase in UT7-Mpl cells requires both Ras-Raf-1- and Rap1-B-Raf-dependent pathways." dans: Molecular and cellular biology, Vol. 21, Issue 8, pp. 2659-70, (2001) (PubMed).

Spaargaren, Bos: "Rab5 induces Rac-independent lamellipodia formation and cell migration." dans: Molecular biology of the cell, Vol. 10, Issue 10, pp. 3239-50, (1999) (PubMed).

Bourne, Sanders, McCormick: "The GTPase superfamily: a conserved switch for diverse cell functions." dans: Nature, Vol. 348, Issue 6297, pp. 125-32, (1990) (PubMed).

Gibbs, Marshall: "The ras oncogene--an important regulatory element in lower eucaryotic organisms." dans: Microbiological reviews, Vol. 53, Issue 2, pp. 171-85, (1989) (PubMed).

Détails sur RAS

Antigène: RAS

Abstract: RAS Produits

Synonymes: anticorps asr, anticorps resistance to audiogenic seizures, anticorps ras

Sujet: Ras and related proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB ) which, upon mutational activation, can function as independent oncogenes. These proteins relay signals from tyrosine kinases at the plasma membrane which subsequently lead to the nucleus via a network of serine/threonine kinases . The p21ras protein is active in its GTP-bound state. This form is slowly converted to the GDP-bound form by the intrinsic GTPase activity of Ras. This activity is greatly enhanced byGTPase-activating proteins (GAPs) which subsequently lead to removal of the GTP molecule and replacement with GDP. Maintenance of Ras in the GTP form can lead to transformation. One class of Ras mutations, commonly found in human tumors, results in an accumulation of Ras-GTP. The mutant Ras can bind GAP, but GAP bound in this manner seems unable to affect the Ras-GTPase activity. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 21 kDa