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C-Raf-1 (AA 162-378) anticorps

Reactivité: Poulet, Chien, Humain, Souris, Rat IF, IP, IHC, WB Hôte: Souris Monoclonal 53-c-Raf unconjugated
N° du produit ABIN967782
  • Antigène
    C-Raf-1
    Épitope
    AA 162-378
    Reactivité
    Poulet, Chien, Humain, Souris, Rat
    Hôte
    • 1
    Souris
    Clonalité
    • 1
    Monoclonal
    Conjugué
    • 1
    Inconjugué
    Application
    Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
     Réactivité croisée
    Chien, Rat (Rattus), Souris, Poulet
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human c-Raf-1 aa. 162-378
    Clone
    53-c-Raf
    Isotype
    IgG1
  • Commentaires

    Related Products: ABIN968533

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20° C.
  • Coles, Shaw: "PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway." dans: Oncogene, Vol. 21, Issue 14, pp. 2236-44, (2002) (PubMed).

    Rizzo, Kraft, Watkins, Levitan, Romero: "Agonist-dependent traffic of raft-associated Ras and Raf-1 is required for activation of the mitogen-activated protein kinase cascade." dans: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 34928-33, (2001) (PubMed).

    Cheng, Wung, Chao, Wang: "Sequential activation of protein kinase C (PKC)-alpha and PKC-epsilon contributes to sustained Raf/ERK1/2 activation in endothelial cells under mechanical strain." dans: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 31368-75, (2001) (PubMed).

    Bondzi, Grant, Krystal: "A novel assay for the measurement of Raf-1 kinase activity." dans: Oncogene, Vol. 19, Issue 43, pp. 5030-3, (2000) (PubMed).

    Rapp: "Role of Raf-1 serine/threonine protein kinase in growth factor signal transduction." dans: Oncogene, Vol. 6, Issue 4, pp. 495-500, (1991) (PubMed).

  • Antigène
    C-Raf-1
    Synonymes
    anticorps CRAF, anticorps NS5, anticorps Raf-1, anticorps c-Raf, anticorps 6430402F14Rik, anticorps AA990557, anticorps BB129353, anticorps Craf1, anticorps D830050J10Rik, anticorps v-Raf, anticorps Raf-1 proto-oncogene, serine/threonine kinase, anticorps v-raf-leukemia viral oncogene 1, anticorps RAF1, anticorps Raf1
    Sujet
    Raf (c-Raf-1) is a cytoplasmic serine/threonine protein kinase and is a member of a family of proteins which are highly conserved from Drosophila to mammals. Raf has a critical role in the response to many growth factors including: EGF, PDGF, insulin, IL-2, IL-3, CSF-1, and GM-CSF. Raf can directly interact with Ras-GTP and subsequently become activated. Thus Raf plays a prominent role in the Ras signaling pathway by transferring a signal from Ras which has been activated by growth factor receptor-stimulated tyrosine kinase activity. This leads to the stimulation and activation of a number of other cytoplasmic serine/threonine kinases. Raf regulates the MAP kinase pathway by phosphorylating and activating MEK, which then phosphorylates and activates MAP kinase (ERK). ERK then phosphorylates and activates Rsk. Raf activity can also be regulated independently of Ras. Cyclic AMP (cAMP) activation of protein kinase A (PKA) can inhibit growth factor stimulation of Raf.
    Poids moléculaire
    74 kDa
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