RAP1 anticorps (AA 1-184)

N° du produit ABIN967814

Détail du produit anti-RAP1 anticorps

Antigène: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Épitope: AA 1-184

Reactivité: Humain, Souris, Rat, Poulet, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Réactivité croisée: Poulet, Grenouille, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Rap1 aa. 1-184

Clone: 3-Rap1

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAP1 (ABIN967814)

Larson, Chen, Kahn, Taylor, Fabre, Mortensen, Conley, Parise: "Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets." dans: Blood, Vol. 101, Issue 4, pp. 1409-15, (2003) (PubMed).

Wu, Lai, Mobley: "Nerve growth factor activates persistent Rap1 signaling in endosomes." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 15, pp. 5406-16, (2001) (PubMed).

Xing, Ge, Zeltser, Maskevitch, Mayer, Alexandropoulos: "c-Src signaling induced by the adapters Sin and Cas is mediated by Rap1 GTPase." dans: Molecular and cellular biology, Vol. 20, Issue 19, pp. 7363-77, (2000) (PubMed).

Okada, Pessin: "Insulin and epidermal growth factor stimulate a conformational change in Rap1 and dissociation of the CrkII-C3G complex." dans: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28179-82, (1997) (PubMed).

Yamamoto, Kaibuchi, Mizuno, Hiroyoshi, Shirataki, Takai: "Purification and characterization from bovine brain cytosol of proteins that regulate the GDP/GTP exchange reaction of smg p21s, ras p21-like GTP-binding proteins." dans: The Journal of biological chemistry, Vol. 265, Issue 27, pp. 16626-34, (1990) (PubMed).

Détails sur RAP1

Antigène: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Autre désignation: Rap1 (TERF2IP Produits)

Synonymes: anticorps DRIP5, anticorps RAP1, anticorps Rap1, anticorps cRAP1, anticorps TERF2 interacting protein, anticorps telomeric repeat binding factor 2, interacting protein, anticorps TERF2IP, anticorps Terf2ip

Sujet: Rap1 is a member of the large Ras superfamily of low molecular weight GTP/GDP binding proteins. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap1A and Rap1B are highly homologous proteins, differing in only 9 of their 184 amino acids. Overexpression of Rap1A (also known as Krev-1) causes reversion of the phenotype of a Ki-Ras-transformed cell line. In vitro, Rap1 can compete efficiently with p21ras for interaction with Ras-GAP. Though they appear to have similar activities, Rap1 and Ras differ in their cellular localization. Ras is found on the inner surface of the plasma membrane while Rap1 is associated with the Golgi. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 21 kDa

Pathways: Cycle Cellulaire, Telomere Maintenance