ZAP70 anticorps (AA 468-619)

N° du produit ABIN967848

Détail du produit anti-ZAP70 anticorps

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Épitope: AA 468-619

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZAP70 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Souris, Rat (Rattus), Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human ZAP-70 Kinase aa. 468-619

Clone: 29-ZAP70 Kinase

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-ZAP70 (ABIN967848)

Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." dans: Immunity, Vol. 15, Issue 6, pp. 935-45, (2001) (PubMed).

Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, (2001) (PubMed).

Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, (2000) (PubMed).

Détails sur ZAP70

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP-70 (ZAP70 Produits)

Synonymes: anticorps SRK, anticorps STCD, anticorps STD, anticorps TZK, anticorps ZAP-70, anticorps 70kDa, anticorps AI327364, anticorps Srk, anticorps mrtle, anticorps mur, anticorps zap70, anticorps MGC80670, anticorps MGC88962, anticorps ZAP70, anticorps zgc:110383, anticorps zeta chain of T-cell receptor associated protein kinase 70, anticorps putative tyrosine-protein kinase zap-70, anticorps zeta-chain (TCR) associated protein kinase, anticorps zeta chain of T cell receptor associated protein kinase 70, anticorps zeta-chain (TCR) associated protein kinase 70kDa, anticorps zeta chain of T cell receptor associated protein kinase 70kDa L homeolog, anticorps zeta chain of T cell receptor associated protein kinase 70kDa, anticorps tyrosine-protein kinase ZAP-70, anticorps ZAP70, anticorps Smp_149470, anticorps Zap70, anticorps zap70.L, anticorps zap70, anticorps LOC707555, anticorps LOC100342021, anticorps LOC100390922, anticorps LOC100542877

Sujet: ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.

Poids moléculaire: 70 kDa

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway