SMARCA2 anticorps (AA 1400-1586)

N° du produit ABIN967938

Détail du produit anti-SMARCA2 anticorps

Antigène: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Épitope: AA 1400-1586

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SMARCA2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Réactivité croisée: Souris, Rat (Rattus), Chien, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Brm aa. 1400-1586

Clone: 24-BRM

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SMARCA2 (ABIN967938)

Wong, Shanahan, Chen, Lian, Ha, Hendricks, Ghaffari, Iliev, Penn, Woodland, Smith, Salada, Carillo, Laity, Gupte, Swedlund, Tavtigian, Teng, Lees: "BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines." dans: Cancer research, Vol. 60, Issue 21, pp. 6171-7, (2000) (PubMed).

Singh, Coe, Hong: "A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor." dans: Nature, Vol. 374, Issue 6522, pp. 562-5, (1995) (PubMed).

Muchardt, Yaniv: "A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor." dans: The EMBO journal, Vol. 12, Issue 11, pp. 4279-90, (1993) (PubMed).

Détails sur SMARCA2

Antigène: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Autre désignation: Brm (SMARCA2 Produits)

Synonymes: anticorps BAF190, anticorps BRM, anticorps NCBRS, anticorps SNF2, anticorps SNF2L2, anticorps SNF2LA, anticorps SWI2, anticorps Sth1p, anticorps hBRM, anticorps hSNF2a, anticorps brm, anticorps 2610209L14Rik, anticorps SNF2alpha, anticorps Snf2l2, anticorps brahma, anticorps psa-4, anticorps wu:fa56c07, anticorps wu:fi27f11, anticorps zgc:66238, anticorps Brahma, anticorps SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2, anticorps SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 L homeolog, anticorps SMARCA2, anticorps smarca2.L, anticorps Smarca2, anticorps smarca2

Sujet: Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. One of these proteins, SNF2/SWI2, has both Drosophila and mammalian homologues, designated brm. The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. The cooperation between Brm and GR requires the DNA binding domain of GR and the helicase domain and the P/Q-charged domain of Brm. However, Brm does not affect on several other transcription factors. The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. Like Brm, Rb up-regulates glucocorticoid-receptor-mediated transcription. Brm and Rb interact in vitro and in vivo, requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 180 kDa