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CDC27 anticorps (AA 145-343)

CDC27 Reactivité: Humain, Rat, Souris, Chien WB, IHC, IP, IF Hôte: Souris Monoclonal 35-CDC27 unconjugated
N° du produit ABIN967980
  • Antigène Voir toutes CDC27 Anticorps
    CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
    Épitope
    • 17
    • 15
    • 7
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 145-343
    Reactivité
    • 65
    • 46
    • 29
    • 9
    • 8
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Souris, Chien
    Hôte
    • 74
    • 7
    Souris
    Clonalité
    • 65
    • 16
    Monoclonal
    Conjugué
    • 35
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp CDC27 est non-conjugé
    Application
    • 30
    • 27
    • 27
    • 22
    • 12
    • 11
    • 9
    • 7
    • 5
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
     Réactivité croisée
    Chien, Rat (Rattus), Souris
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human CDC27 aa. 145-343
    Clone
    35-CDC27
    Isotype
    IgG2b
    Top Product
    Discover our top product CDC27 Anticorps primaire
  • Commentaires

    Related Products: ABIN968535, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20° C.
  • Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

    Kramer, Scheuringer, Podtelejnikov, Mann, Peters: "Mitotic regulation of the APC activator proteins CDC20 and CDH1." dans: Molecular biology of the cell, Vol. 11, Issue 5, pp. 1555-69, (2000) (PubMed).

    Tugendreich, Boguski, Seldin, Hieter: "Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 21, pp. 10031-5, (1993) (PubMed).

  • Antigène
    CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
    Autre désignation
    Cdc27 (CDC27 Produits)
    Synonymes
    anticorps ANAPC3, anticorps APC3, anticorps CDC27Hs, anticorps D0S1430E, anticorps D17S978E, anticorps HNUC, anticorps NUC2, anticorps wu:fb54g06, anticorps wu:fc88c12, anticorps AI452358, anticorps BC023187, anticorps CDC27, anticorps NV10210, anticorps cell division cycle 27, anticorps cell division cycle 27 S homeolog, anticorps cell division cycle protein 27 homolog, anticorps anaphase-promoting complex subunit Apc3, anticorps CDC27, anticorps cdc27, anticorps Cdc27, anticorps cdc27.S, anticorps LOC100123129, anticorps nuc2
    Sujet
    CDC16 and CDC27 were discovered in yeast as genes essential for the G2/M transition of the cell cycle. The human homologues of CDC16 and CDC27 were cloned and found to encode proteins of 72kDa and 97kDa, respectively. Biochemical and subcellular localization experiments have shown that CDC16 and CDC27 are novel components of the mitotic spindle centrosome in human cells. In HeLa cells, there are about 100,000 copies of each protein per cell. Although its precise role during mitosis remains to be determined, CDC16 appears to be required for cyclin degradation in yeast. Wild type CDC27 is required for the initiation of anaphase. Immunofluorescence studies have shown that both proteins are components of the centrosome in the absence of spindle fibers.
    Poids moléculaire
    97 kDa
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