Ron alpha (AA 40-224) anticorps

N° du produit ABIN968150

Détail du produit

Antigène: Ron alpha

Épitope: AA 40-224

Reactivité: Humain, Chien, Grenouille, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Réactivité croisée: Chien, Rat (Rattus), Souris, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human RONalpha aa. 40-224

Clone: 29-RONalpha

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN968619, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Référence

Peace, Hughes, Degen, Waltz: "Point mutations and overexpression of Ron induce transformation, tumor formation, and metastasis." dans: Oncogene, Vol. 20, Issue 43, pp. 6142-51, (2001) (PubMed).

Gaudino, Follenzi, Naldini, Collesi, Santoro, Gallo, Godowski, Comoglio: "RON is a heterodimeric tyrosine kinase receptor activated by the HGF homologue MSP." dans: The EMBO journal, Vol. 13, Issue 15, pp. 3524-32, (1994) (PubMed).

Wang, Ronsin, Gesnel, Coupey, Skeel, Leonard, Breathnach: "Identification of the ron gene product as the receptor for the human macrophage stimulating protein." dans: Science (New York, N.Y.), Vol. 266, Issue 5182, pp. 117-9, (1994) (PubMed).

Ronsin, Muscatelli, Mattei, Breathnach: "A novel putative receptor protein tyrosine kinase of the met family." dans: Oncogene, Vol. 8, Issue 5, pp. 1195-202, (1993) (PubMed).

Détail du antigène

Antigène: Ron alpha

Sujet: RON (Recepteur d'Origine Nantaise) is a tyrosine kinase receptor for MSP (Macrophage Stimulating Protein) that belongs to the hepatocyte growth factor receptor family. RON is synthesized as a single chain glycosylated polypeptide of 1400 amino acids that is cleaved upon maturation into a 150 kDa beta-subunit with the kinase activity and a 40 kDa alpha-chain. Both chains are linked by disulfide bonds and the proper arrangement of the alpha and beta heterodimer is necessary for biological activity of RON. MSP induces the tyrosine kinase activity of RON, triggering a cascade of intracellular signaling that leads to DNA synthesis. However, RON activation in mouse erythroleukemia cells results in apoptosis. The replacement of autophosphorylation sites Y1330 and Y1337 abolishes both the mitogenic and apoptotic effects of MSP. Therefore, autophosphorylation at Y1330 and Y1337 is required for RON's biological activity. PLCgamma, Shc, Grb2, p61, and p65 associate with RON. Although widely expressed, RON is particularly abundant in epithelial tissues, lung, brain, adrenal glands, gastrointestinal tract, kidney, and testis.
Synonyms: Recepteur d'Origine Nantaise

Poids moléculaire: 40 kDa