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CPE anticorps (AA 49-200)

CPE Reactivité: Humain, Souris, Rat WB, IHC, IP, IF Hôte: Souris Monoclonal 35-Carboxypeptidase E unconjugated
N° du produit ABIN968159
  • Antigène Voir toutes CPE Anticorps
    CPE (Carboxypeptidase E (CPE))
    Épitope
    • 15
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 49-200
    Reactivité
    • 48
    • 28
    • 26
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 44
    • 3
    • 1
    Souris
    Clonalité
    • 45
    • 3
    Monoclonal
    Conjugué
    • 24
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CPE est non-conjugé
    Application
    • 39
    • 19
    • 13
    • 13
    • 12
    • 8
    • 6
    • 5
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
     Réactivité croisée
    Rat (Rattus), Souris
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human Carboxypeptidase E aa. 49-200
    Clone
    35-Carboxypeptidase E
    Isotype
    IgG1
    Top Product
    Discover our top product CPE Anticorps primaire
  • Indications d'application
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    Commentaires

    Related Products: ABIN968545

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20° C.
  • Cool, Normant, Shen, Chen, Pannell, Zhang, Loh: "Carboxypeptidase E is a regulated secretory pathway sorting receptor: genetic obliteration leads to endocrine disorders in Cpe(fat) mice." dans: Cell, Vol. 88, Issue 1, pp. 73-83, (1997) (PubMed).

    Shen, Loh: "Intracellular misrouting and abnormal secretion of adrenocorticotropin and growth hormone in cpefat mice associated with a carboxypeptidase E mutation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5314-9, (1997) (PubMed).

    Varlamov, Fricker: "The C-terminal region of carboxypeptidase E involved in membrane binding is distinct from the region involved with intracellular routing." dans: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6077-83, (1996) (PubMed).

    Manser, Fernandez, Loo, Goh, Monfries, Hall, Lim: "Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro." dans: The Biochemical journal, Vol. 267, Issue 2, pp. 517-25, (1990) (PubMed).

  • Antigène
    CPE (Carboxypeptidase E (CPE))
    Autre désignation
    Carboxypeptidase E (CPE Produits)
    Synonymes
    anticorps CPE, anticorps CPH, anticorps Cph-1, anticorps Cph1, anticorps R74677, anticorps fat, anticorps CARBE, anticorps Cph, anticorps si:ch211-198g9.2, anticorps zgc:85981, anticorps carboxypeptidase E, anticorps carboxypeptidase E L homeolog, anticorps CPE, anticorps cpe, anticorps Cpe, anticorps cpe.L
    Sujet
    Carboxypeptidase E (CPE), also known as carboxypeptidase H and enkephalin convertase, is found as both a membrane-bound and a soluble glycoprotein in neuroendocrine tissues and adrenal-gland chromaffin granules. The C-terminus forms an amphiphilic alpha-helix, suggesting that this region is responsible for the membrane-bound form. Evidence suggests the active form of CPE is located in the secretory vesicles. CPE appears to have several functions. It is an exopeptidase that cleaves neuropeptides with C-terminal basic amino acids, producing an active form of the peptide. It has also been proposed that membrane-bound CPE is a sorting receptor for regulated secretory pathway (RSP) proteins in the TGN pituitary Golgi and secretory granule membranes. RSP proteins primarily consist of hormones and neuropeptides. Mice that carry a mutation in the CPE gene Cpe[fat] display endocrine disorders such as obesity, infertility, and hyperproinsulinemia. Furthermore, the same endocrine disorders are observed in Cpe[fat] mice where the CPE gene has been effaced by antisense RNA.
    Poids moléculaire
    50 kDa
    Pathways
    Peptide Hormone Metabolism, Synaptic Membrane
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