EPS15 anticorps (AA 694-888)

N° du produit ABIN968187

Détail du produit anti-EPS15 anticorps

Antigène: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Épitope: AA 694-888

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EPS15 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Eps15 aa. 694-888

Clone: 17-eps15

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-EPS15 (ABIN968187)

Engqvist-Goldstein, Warren, Kessels, Keen, Heuser, Drubin: "The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro." dans: The Journal of cell biology, Vol. 154, Issue 6, pp. 1209-23, (2001) (PubMed).

Kariya, Koyama, Nakashima, Oshiro, Morinaka, Kikuchi: "Regulation of complex formation of POB1/epsin/adaptor protein complex 2 by mitotic phosphorylation." dans: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18399-406, (2000) (PubMed).

Traub, Downs, Westrich, Fremont: "Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 16, pp. 8907-12, (1999) (PubMed).

Iannolo, Salcini, Gaidarov, Goodman, Baulida, Carpenter, Pelicci, Di Fiore, Keen: "Mapping of the molecular determinants involved in the interaction between eps15 and AP-2." dans: Cancer research, Vol. 57, Issue 2, pp. 240-5, (1997) (PubMed).

Alvarez, Shon, Miloso, Beguinot: "Structural requirements of the epidermal growth factor receptor for tyrosine phosphorylation of eps8 and eps15, substrates lacking Src SH2 homology domains." dans: The Journal of biological chemistry, Vol. 270, Issue 27, pp. 16271-6, (1995) (PubMed).

Détails sur EPS15

Antigène: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Autre désignation: Eps15 (EPS15 Produits)

Synonymes: anticorps AF-1P, anticorps AF1P, anticorps MLLT5, anticorps CG16932, anticorps Dmel\\CG16932, anticorps EH1, anticorps EPS-15, anticorps Eps15, anticorps anon-WO0118547.85, anticorps eps-15, anticorps eps15, anticorps i31, anticorps 2410112D09Rik, anticorps RGD1307641, anticorps epidermal growth factor receptor pathway substrate 15, anticorps Epidermal growth factor receptor pathway substrate clone 15, anticorps hypothetical protein, anticorps EPS15, anticorps Eps-15, anticorps eps15, anticorps Eps15, anticorps PGTG_06574

Sujet: The eps15 (EGF receptor pathway substrate clone number 15) gene was isolated using an expression cloning method designed for direct isolation of cDNAs encoding substrates of tyrosine kinases. The eps15 gene encodes a 145 kDa protein that is tyrosine phosphorylated following EGF receptor activation and this receptor directly phosphorylates purified Eps15 in vitro. Phosphorylation of Eps15 is much more efficient following EGF receptor activation versus erbB2 kinase activation, suggesting the protein is predominantly a part of an EGF receptor signaling pathway. Overexpression of Eps15 causes transformation of NIH 3T3 cells, implying Eps15 may be involved in the control of cell proliferation. Eps15 binds to the SH3 domain of Crk through its proline-rich domain at the C-terminal region. Adaptin alpha binds to the C-terminus of Eps15 at three different sites ranging from residues 650 to 730. Eps15, Adaptin alpha, and EGF receptor are co-localized at the clathrin-coated pits after receptor activation. Therefore, Eps15 may recruit EGF receptors to the clathrin-coated pits for internalization of the activated receptor. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 145 kDa

Pathways: EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation