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p62 Lck Ligand (AA 257-437) anticorps

Reactivité: Humain, Souris, Rat IF, IP, IHC, WB Hôte: Souris Monoclonal 3-P62 LCK LIGAND unconjugated
N° du produit ABIN968203
  • Antigène
    p62 Lck Ligand
    Épitope
    AA 257-437
    Reactivité
    Humain, Souris, Rat
    Hôte
    • 1
    Souris
    Clonalité
    • 1
    Monoclonal
    Conjugué
    • 1
    Inconjugué
    Application
    Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
     Réactivité croisée
    Souris, Rat (Rattus)
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human p62 lck ligand aa. 257-437
    Clone
    3-P62 LCK LIGAND
    Isotype
    IgG1
  • Commentaires

    Related Products: ABIN968551, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Cariou, Perdereau, Cailliau, Browaeys-Poly, Béréziat, Vasseur-Cognet, Girard, Burnol: "The adapter protein ZIP binds Grb14 and regulates its inhibitory action on insulin signaling by recruiting protein kinase Czeta." dans: Molecular and cellular biology, Vol. 22, Issue 20, pp. 6959-70, (2002) (PubMed).

    Puls, Schmidt, Grawe, Stabel: "Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6191-6, (1997) (PubMed).

  • Antigène
    p62 Lck Ligand
    Sujet
    P62 lck ligand (zeta-interacting protein (ZIP)) is a cytoplasmic protein that binds to the SH2 domain of lck (a T cell src tyrosine kinase) in the absence of a phosphotyrosine in either protein. The ubiquitously expressed p62 lck ligand contains a cysteine rich region that is similar to a zinc finger domain, a G protein binding region, a PEST sequence, and several phosphorylation sites. Deletion of the p62 lck ligand N-terminal domain has been reported to abrogate its binding to lck. However, mutation of the tyrosine did not have an effect. In addition, p62 lck ligand binds to the pseudosubstrate region of the PKCzeta catalytic domain. In turn, PKCzeta phosphorylates p62. p62 lck ligand binds to the dimerization region of PKCzeta, thereby inhibiting PKCzeta-PKCzeta interaction. This suggests that p62 lck ligand may compete with PKCzeta. However, it requires PKCzeta for proper subcellular localization. These data suggest that p62 lck ligand may be part of the protein bridge that links PKCzeta to the tyrosine kinases involved in signaling pathways. This antibody is routinely tested by western blot analysis.
    Synonyms: Zeta Interacting Protein (ZIP)
    Poids moléculaire
    62 kDa
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