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NF-kB p65 anticorps (AA 136-224)

NFkBP65 Reactivité: Humain, Rat, Chien, Lapin, Grenouille WB, BI Hôte: Souris Monoclonal 20-NF unconjugated
N° du produit ABIN968222
  • Antigène Voir toutes NF-kB p65 (NFkBP65) Anticorps
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    Épitope
    • 44
    • 32
    • 29
    • 28
    • 28
    • 25
    • 23
    • 23
    • 20
    • 17
    • 16
    • 15
    • 14
    • 11
    • 9
    • 9
    • 8
    • 8
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
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    • 2
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    AA 136-224
    Reactivité
    • 540
    • 374
    • 301
    • 41
    • 38
    • 29
    • 24
    • 21
    • 18
    • 16
    • 14
    • 11
    • 5
    • 2
    • 1
    • 1
    • 1
    Humain, Rat, Chien, Lapin, Grenouille
    Hôte
    • 492
    • 52
    • 4
    • 2
    • 1
    Souris
    Clonalité
    • 458
    • 92
    Monoclonal
    Conjugué
    • 295
    • 30
    • 23
    • 19
    • 18
    • 17
    • 17
    • 17
    • 17
    • 17
    • 16
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 2
    • 2
    • 1
    Cet anticorp NF-kB p65 est non-conjugé
    Application
    • 427
    • 203
    • 135
    • 118
    • 112
    • 104
    • 92
    • 78
    • 76
    • 29
    • 25
    • 9
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), BioImaging (BI)
     Réactivité croisée
    Chien, Grenouille, Lapin, Rat (Rattus)
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    6. Triton is a trademark of the Dow Chemical Company.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human NF-kappaB aa. 136-224
    Clone
    20-NF
    Isotype
    IgG1
    Top Product
    Discover our top product NFkBP65 Anticorps primaire
  • Indications d'application
    Bioimaging
    1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
    2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
    3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
    4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
    5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
    6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
    7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
    8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
    9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
    10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
    11. View and analyze the cells on an appropriate imaging instrument.
    Commentaires

    Related Products: ABIN968537, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Nishibe, Parry, Ishida, Aziz, Murray, Patel, Rahman, Strand, Saito, Saito, Hammond, Savidge, Mackman, Wijelath: "Oncostatin M promotes biphasic tissue factor expression in smooth muscle cells: evidence for Erk-1/2 activation." dans: Blood, Vol. 97, Issue 3, pp. 692-9, (2001) (PubMed).

    Pimentel-Muiños, Seed: "Regulated commitment of TNF receptor signaling: a molecular switch for death or activation." dans: Immunity, Vol. 11, Issue 6, pp. 783-93, (2000) (PubMed).

    Kieran, Blank, Logeat, Vandekerckhove, Lottspeich, Le Bail, Urban, Kourilsky, Baeuerle, Israël: "The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product." dans: Cell, Vol. 62, Issue 5, pp. 1007-18, (1990) (PubMed).

    Shirakawa, Mizel: "In vitro activation and nuclear translocation of NF-kappa B catalyzed by cyclic AMP-dependent protein kinase and protein kinase C." dans: Molecular and cellular biology, Vol. 9, Issue 6, pp. 2424-30, (1989) (PubMed).

    Baeuerle, Baltimore: "Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor." dans: Cell, Vol. 53, Issue 2, pp. 211-7, (1988) (PubMed).

  • Antigène
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    Autre désignation
    NF-kappa B p65 (NFkBP65 Produits)
    Synonymes
    anticorps C-Rel, anticorps NFKB3, anticorps p65, anticorps NFkB, anticorps c-rel, anticorps zgc:100833, anticorps Xrel2, anticorps Xrel3, anticorps rel, anticorps rel-A, anticorps rel2, anticorps rel3, anticorps v-rel, anticorps xrel, anticorps REL proto-oncogene, NF-kB subunit, anticorps RELA proto-oncogene, NF-kB subunit, anticorps v-rel reticuloendotheliosis viral oncogene homolog A (avian), anticorps v-rel avian reticuloendotheliosis viral oncogene homolog, anticorps v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog, anticorps REL, anticorps RELA, anticorps Rela, anticorps Rel, anticorps rel, anticorps rel.L
    Sujet
    NF-kappaB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. The most studied NF-kappaB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-kappaB with its inhibitor, IkappaB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkappaB. This complex prevents nuclear translocation and activity of NF-kappaB. In response to stimuli such as cytokines, LPS, and viral infections, IkappaB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkappaB/NF-kappaB complex, allowing the free heterodimeric NF-kappaB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the kappaB site within promoters and enhancers and functions as a transcriptional activator.
    Poids moléculaire
    65 kDa
    Pathways
    Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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