RAD23B anticorps (AA 73-193)

N° du produit ABIN968319

Détail du produit anti-RAD23B anticorps

Antigène: RAD23B (RAD23 Homolog B (RAD23B))

Épitope: AA 73-193

Reactivité: Humain, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAD23B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human hHR23B aa. 73-193

Clone: 16-hHR23B

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAD23B (ABIN968319)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Wakasugi, Sancar: "Assembly, subunit composition, and footprint of human DNA repair excision nuclease." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 12, pp. 6669-74, (1998) (PubMed).

Reardon, Mu, Sancar: "Overproduction, purification, and characterization of the XPC subunit of the human DNA repair excision nuclease." dans: The Journal of biological chemistry, Vol. 271, Issue 32, pp. 19451-6, (1996) (PubMed).

Sugasawa, Masutani, Uchida, Maekawa, van der Spek, Bootsma, Hoeijmakers, Hanaoka: "HHR23B, a human Rad23 homolog, stimulates XPC protein in nucleotide excision repair in vitro." dans: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4852-61, (1996) (PubMed).

Masutani, Sugasawa, Yanagisawa, Sonoyama, Ui, Enomoto, Takio, Tanaka, van der Spek, Bootsma: "Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23." dans: The EMBO journal, Vol. 13, Issue 8, pp. 1831-43, (1994) (PubMed).

Détails sur RAD23B

Antigène: RAD23B (RAD23 Homolog B (RAD23B))

Autre désignation: hHR23B (RAD23B Produits)

Synonymes: anticorps HHR23B, anticorps HR23B, anticorps P58, anticorps 0610007D13Rik, anticorps AV001138, anticorps mHR23B, anticorps p58, anticorps MGC107846, anticorps zgc:65951, anticorps RAD23 homolog B, nucleotide excision repair protein, anticorps UV excision repair protein RAD23 homolog B, anticorps RAD23 homolog B, nucleotide excision repair protein S homeolog, anticorps RAD23B, anticorps Rad23b, anticorps rd23b, anticorps rad23b, anticorps rad23b.S

Sujet: Nucleotide excision repair (NER) is an essential element of DNA repair which eliminates unrelated DNA base lesions, including those induced by UV irradiation or chemicals. Although well defined in prokaryotes, eukaryotic NER is complex and involves at least two distinct subpathways. The transcription-coupled repair subpathway eliminates DNA lesions just prior to transcription. This is important for the removal of lesions for which the second pathway, global genome repair (i.e. covers the entire genome), is unable to repair. Individuals with defects in both pathways may suffer from xeroderma pigmentosum (XP), a condition with increased incidence of cancer in skin and internal organs. Mutations in seven genes result in human XP. However, one of these, XP-C, affects only the global genome repair pathway. The XP-C protein interacts with hHR23B, the human homolog of RAD23, an essential component of yeast NER. The XPC-hHR23B complex is required for excision of thymine dimers. In addition, hHR23B contains an N-terminal ubiquitin-like domain that allows for proteasome interaction. Thus, hHR23B may be an essential component of NER and mechanisms of DNA repair. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 58 kDa

Pathways: Réparation de l'ADN