PARP1 anticorps (AA 22-219)

N° du produit ABIN968333

Détail du produit anti-PARP1 anticorps

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Épitope: AA 22-219

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PARP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris, Rat (Rattus), Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human PARP aa. 22-219

Clone: 42-PARP

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-PARP1 (ABIN968333)

Jaiswal, Marlow, Gupta, Narayan: "Beta-catenin-mediated transactivation and cell-cell adhesion pathways are important in curcumin (diferuylmethane)-induced growth arrest and apoptosis in colon cancer cells." dans: Oncogene, Vol. 21, Issue 55, pp. 8414-27, (2002) (PubMed).

Liao, Chien, Shenoy, Mendel, McMahon, Cherrington, London: "Inhibition of constitutively active forms of mutant kit by multitargeted indolinone tyrosine kinase inhibitors." dans: Blood, Vol. 100, Issue 2, pp. 585-93, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Duriez, Shah: "Cleavage of poly(ADP-ribose) polymerase: a sensitive parameter to study cell death." dans: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 75, Issue 4, pp. 337-49, (1998) (PubMed).

Cherney, McBride, Chen, Alkhatib, Bhatia, Hensley, Smulson: "cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 23, pp. 8370-4, (1988) (PubMed).

Détails sur PARP1

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: PARP (PARP1 Produits)

Synonymes: anticorps ADPRT, anticorps ADPRT 1, anticorps ADPRT1, anticorps ARTD1, anticorps PARP, anticorps PARP-1, anticorps PPOL, anticorps pADPRT-1, anticorps Adprt, anticorps Parp-1, anticorps 5830444G22Rik, anticorps AI893648, anticorps Adprp, anticorps Adprt1, anticorps C80510, anticorps parp-1, anticorps sPARP-1, anticorps BEST:LD21673, anticorps CG17685, anticorps CG17696, anticorps CG17718, anticorps CG40411, anticorps D.PARP, anticorps Dm.pARTa, anticorps Dmel\\CG40411, anticorps LD21673.3prime, anticorps PARP1, anticorps dPARP, anticorps parp, anticorps adprt1, anticorps padprt-1, anticorps ppol, anticorps si:dkey-206f10.3, anticorps wu:fc60f12, anticorps zgc:110092, anticorps poly(ADP-ribose) polymerase 1, anticorps poly (ADP-ribose) polymerase 1, anticorps poly (ADP-ribose) polymerase family, member 1, anticorps Poly-(ADP-ribose) polymerase, anticorps poly(ADP-ribose) polymerase 1 L homeolog, anticorps PARP1, anticorps Parp1, anticorps Parp, anticorps parp1.L, anticorps parp1

Sujet: Poly(ADP-ribose) polymerase (PARP) is a constitutively expressed, abundant nuclear protein. It has been referred to as a molecular nick sensor due to its recognition of, and catalytic activation by, single or double strand DNA breaks. The most critical ad extensively studied role of PARP is its participation in DNA base excision repair. Following binding to damaged DNA, PARP uses NAD+ to synthesize branched polymers of poly(ADP-ribose) on nuclear target proteins, including itself. Such modification of PARP increases its negative charge and results in loss of interactionwith DNA due to electrostatic repulsion. This opens the damaged DNA to DNA repair proteins. The poly(ADP-ribose) molecule is quickly degraded by poly (ADP-ribose) glycohydrolase that is found in association with PARP. PARP contains N-terminal DNA-binding domain (DBD), a central automodification domain that accepts poly (ADP-ribose), and a C-terminal catalytic domain. PARP is one of the earliest proteins targeted by caspase-3 during apoptosis. Although this protein is central to DNA repair, it has additional DNA-related functions that remain to be investigated. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 113 kDa

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location