LAMP1 anticorps (AA 25-224)

N° du produit ABIN968337

Détail du produit anti-LAMP1 anticorps

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Épitope: AA 25-224

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Lamp-1 aa. 25-224

Clone: 25-Lamp

Isotype: IgG2b

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-LAMP1 (ABIN968337)

Alto, Soderling, Scott: "Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics." dans: The Journal of cell biology, Vol. 158, Issue 4, pp. 659-68, (2002) (PubMed).

Hocking, Kowalski: "A cryptic fragment from fibronectin's III1 module localizes to lipid rafts and stimulates cell growth and contractility." dans: The Journal of cell biology, Vol. 158, Issue 1, pp. 175-84, (2002) (PubMed).

Spoerl, Stumpf, Noegel, Hasse: "Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus." dans: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48858-67, (2002) (PubMed).

Fukuda: "Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking." dans: The Journal of biological chemistry, Vol. 266, Issue 32, pp. 21327-30, (1991) (PubMed).

Fukuda, Viitala, Matteson, Carlsson: "Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences." dans: The Journal of biological chemistry, Vol. 263, Issue 35, pp. 18920-8, (1989) (PubMed).

Détails sur LAMP1

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Autre désignation: Lamp-1 (LAMP1 Produits)

Synonymes: anticorps CD107a, anticorps LAMPA, anticorps LGP120, anticorps AI196048, anticorps Lamp-1, anticorps LEP100, anticorps LAMP-1, anticorps LGP-A, anticorps Lgpa, anticorps Lysosome-associated membrane protein 1, anticorps cd107a, anticorps lamp-1, anticorps lamp1, anticorps lampa, anticorps lgp120, anticorps rexp76, anticorps lysosomal associated membrane protein 1, anticorps lysosomal-associated membrane protein 1, anticorps lysosomal-associated membrane protein 1 L homeolog, anticorps LAMP1, anticorps Lamp1, anticorps lamp1.L

Sujet: Lysosomes are digestive compartments in mammalian cells that are responsible for the degradation of numerous substances including foreign materials. The lysosomal membrane is of interest due to its role in maintaining the acidic intralysosomal sac, its resistance to degradation, and its ability to fuse with other membranous organelles. Lamp-1 and lamp-2 (lysosomal-associated membrane proteins -1 and -2) were identified as lysosomal membrane proteins that act as carriers for poly-N-glycans, primarily N-acetyllactosamines. They consist of two heavily glycosylated amino terminal domains, a single trans-membrane domain, and a short cytoplasmic tail. A tyrosine located at the fourth residue from the end of the cytoplasmic tail, a large hydrophobic amino acid as the last residue, and a glycine residue amino terminal to the tyrosine residue targets these proteins from the TGN to the lysosome. The role of the Lamp molecules may be a carrier for poly-N-acetyllactosamines and/or to protect lysosomal membranes from the soluble hydrolases. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 110 kDa

Pathways: Autophagy