PDPK1 anticorps (3-phosphoinositide Dependent Protein Kinase-1) (AA 439-555)

Details for Product anti-PDPK1 Antibody No. ABIN968351, Fournisseur: Connectez-vous pour afficher
Antigène
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • zgc:153787
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase-1a
  • 3-phosphoinositide-dependent protein kinase 1
  • Phosphoinositide-dependent kinase 1
  • Protein PDK-1
  • PDPK1
  • pdpk1
  • Pdpk1
  • pdpk1a
  • Pdk1
  • pdk-1
Épitope
AA 439-555
59
35
32
27
27
26
18
17
15
15
15
15
7
7
6
6
6
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Chien, Humain, Souris, Rat (Rattus)
464
220
220
19
13
12
11
10
6
5
4
3
2
1
1
1
1
1
1
1
Hôte
Souris
384
72
16
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp PDPK1 est non-conjugé
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
313
158
102
91
67
65
40
31
22
10
7
5
3
2
1
Options
Fournisseur
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Supplier Product No.
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Immunogène Rat PDPK1 aa. 439-555
Clone 5-PDK1
Isotype IgG1
 Réactivité croisée Humain, Chien, Souris
Attributs du produit 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Autre désignation PDK1 (PDPK1 Antibody Extrait)
Sujet Protein Kinase B (PKB) is downstream of phosphoinositide 3-kinase (PI3-Kinase) that functions in a critical signaling pathway for receptor-dependent cell activation. PKB activation requires the binding of lipid metabolites phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate, phospholipid products of PI3-Kinase. These lipids bind to PKB and induce a conformational change in the enzyme, resulting in its translocation to the plasma membrane. Following cell activation, PKB is phosphorylated on Thr-308 by another kinase designated as 3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) or also known as PKB Kinase. This kinase probably represents a new family of phospholipid-dependent activities with molecular weights that range from 31 to 220 kDa. PDPK1 consists of 559 amino acids with an N-terminal catalytic domain and a pleckstrin-homology (PH) domain located in the C-terminal region. Like PKB, PDPK1 requires the presence of phosphatidylinositol 3,4,5-trisphosphate for maximal activity. Thus, PDPK1 acts upstream of PKB and may control signals important for proliferation, apoptosis, and metabolism. This antibody is routinely tested by western blot analysis.
Synonyms: PKB Kinase, Akt Kinase, PDK1
Poids moléculaire 58-63 kDa
Domaine de recherche Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways Signalisation PI3K-Akt, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Commentaires

Related Products: ABIN968552, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 439-555) antibody (ABIN968351) Western blot analysis of PDPK1 on a SW-13 cell lysate (human adrenal gland carcinoma,...
Produit citée dans: Fujita, Sato, Ishida, Tsuruo: "Involvement of Hsp90 in signaling and stability of 3-phosphoinositide-dependent kinase-1." dans: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10346-53, 2002 (PubMed).

Scheid, Marignani, Woodgett: "Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B." dans: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6247-60, 2002 (PubMed).

Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." dans: Cancer research, Vol. 60, Issue 18, pp. 5303-9, 2000 (PubMed).

Klippel, Kavanaugh, Pot, Williams: "A specific product of phosphatidylinositol 3-kinase directly activates the protein kinase Akt through its pleckstrin homology domain." dans: Molecular and cellular biology, Vol. 17, Issue 1, pp. 338-44, 1997 (PubMed).

Stokoe, Stephens, Copeland, Gaffney, Reese, Painter, Holmes, McCormick, Hawkins: "Dual role of phosphatidylinositol-3,4,5-trisphosphate in the activation of protein kinase B." dans: Science (New York, N.Y.), Vol. 277, Issue 5325, pp. 567-70, 1997 (PubMed).

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