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ROCK1 anticorps (AA 906-1012)

ROCK1 Reactivité: Humain, Souris, Rat WB, IF Hôte: Souris Monoclonal 46-ROCK unconjugated
N° du produit ABIN968387
  • Antigène Voir toutes ROCK1 Anticorps
    ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
    Épitope
    • 16
    • 16
    • 15
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 906-1012
    Reactivité
    • 79
    • 59
    • 57
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 71
    • 9
    Souris
    Clonalité
    • 58
    • 22
    Monoclonal
    Conjugué
    • 39
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp ROCK1 est non-conjugé
    Application
    • 69
    • 27
    • 26
    • 20
    • 17
    • 15
    • 13
    • 12
    • 11
    • 8
    • 6
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
     Réactivité croisée
    Humain, Rat (Rattus)
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Mouse ROCK-I aa. 906-1012
    Clone
    46-ROCK
    Isotype
    IgG1
    Top Product
    Discover our top product ROCK1 Anticorps primaire
  • Commentaires

    Related Products: ABIN967389, ABIN968542

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Pawlak, Helfman: "Post-transcriptional down-regulation of ROCKI/Rho-kinase through an MEK-dependent pathway leads to cytoskeleton disruption in Ras-transformed fibroblasts." dans: Molecular biology of the cell, Vol. 13, Issue 1, pp. 336-47, (2002) (PubMed).

    Wang, Eto, Steers, Somlyo, Somlyo: "RhoA-mediated Ca2+ sensitization in erectile function." dans: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, (2002) (PubMed).

    Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." dans: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, (2001) (PubMed).

    Ishizaki, Maekawa, Fujisawa, Okawa, Iwamatsu, Fujita, Watanabe, Saito, Kakizuka, Morii, Narumiya: "The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase." dans: The EMBO journal, Vol. 15, Issue 8, pp. 1885-93, (1996) (PubMed).

    Nakagawa, Fujisawa, Ishizaki, Saito, Nakao, Narumiya: "ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice." dans: FEBS letters, Vol. 392, Issue 2, pp. 189-93, (1996) (PubMed).

  • Antigène
    ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
    Autre désignation
    ROCK-I (ROCK1 Produits)
    Synonymes
    anticorps LOC100229794, anticorps CG9774, anticorps DROK, anticorps DRhk, anticorps DRok, anticorps Dmel\\CG9774, anticorps Drok, anticorps ROCK, anticorps ROCK-1, anticorps ROCK1, anticorps ROK, anticorps ROKalpha/beta, anticorps Rhk, anticorps RhoK, anticorps Rock, anticorps Rok, anticorps dROK, anticorps dRok, anticorps drok, anticorps 1110055K06Rik, anticorps Rock-I, anticorps P160Rock, anticorps ROCK-I, anticorps P160ROCK, anticorps p160ROCK, anticorps Rho associated coiled-coil containing protein kinase 1, anticorps Rho kinase, anticorps Rho-associated coiled-coil containing protein kinase 1, anticorps Rho-associated, coiled-coil containing protein kinase 1, anticorps ROCK1, anticorps Rok, anticorps Rock1, anticorps rock1
    Sujet
    ROCK-I is a Rho-associated serine/threonine kinase isozyme that mediates RhoA-induced assembly of focal adhesions and actin stress fibers. It contains an N-terminal kinase domain, a central 600 amino acid long coiled-coil region, a C-terminal pleckstrin homology region (PH) and a Cys-rich zinc finger motif. The ROCK-I kinase domain is approximately 90% identical to that of ROCK-II. ROCK-I binds GTP-bound Rho through a Rho-binding domain (RBD). As a result, the kinase activity of ROCK-I is moderately stimulated. The ROCK isozymes regulate cell contractility through phosphorylation of the myosin light chain. This effect results from either the inhibition of the myosin phosphatase or by direct phosphorylation of the myosin light chain, thus bypassing the myosin light chain kinase. In addition, ROCK-I activates the ubiquitously expressed Na-H exchanger (NHE1) via a number of mechanisms including RhoA. NHE1 may mediate ROCK-I-induced changes in the actin cytoskeleton. Therefore, ROCK-I plays an important role in the regulation of focal adhesion and stress fiber formation.
    Synonyms: ROKbeta
    Poids moléculaire
    160 kDa
    Pathways
    Dynamique des Microtubules, Signalisation WNT, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
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