SATB1 anticorps (AA 550-667)

N° du produit ABIN968403

Détail du produit anti-SATB1 anticorps

Antigène: SATB1 (SATB Homeobox 1 (SATB1))

Épitope: AA 550-667

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SATB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human SATB1 aa. 550-667

Clone: 14-SATB1

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-SATB1 (ABIN968403)

Skowronska-Krawczyk, Ma, Schwartz, Scully, Li, Liu, Taylor, Tollkuhn, Ohgi, Notani, Kohwi, Kohwi-Shigematsu, Rosenfeld: "Required enhancer-matrin-3 network interactions for a homeodomain transcription program." dans: Nature, Vol. 514, Issue 7521, pp. 257-61, (2014) (PubMed).

Beyer, Thabet, Müller, Sadlon, Classen, Lahl, Basu, Zhou, Bailey-Bucktrout, Krebs, Schönfeld, Böttcher, Golovina, Mayer, Hofmann, Sommer, Debey-Pascher, Endl, Limmer, Hippen, Blazar, Balderas, Quast et al.: "Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. ..." dans: Nature immunology, Vol. 12, Issue 9, pp. 898-907, (2011) (PubMed).

Alvarez, Yasui, Niida, Joh, Loh, Kohwi-Shigematsu: "The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development." dans: Genes & development, Vol. 14, Issue 5, pp. 521-35, (2000) (PubMed).

Cai, Kohwi-Shigematsu: "Intranuclear relocalization of matrix binding sites during T cell activation detected by amplified fluorescence in situ hybridization." dans: Methods (San Diego, Calif.), Vol. 19, Issue 3, pp. 394-402, (2000) (PubMed).

de Belle, Cai, Kohwi-Shigematsu: "The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops." dans: The Journal of cell biology, Vol. 141, Issue 2, pp. 335-48, (1998) (PubMed).

Liu, Bramblett, Zhu, Lozano, Kobayashi, Ross, Dudley: "The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression." dans: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5275-87, (1997) (PubMed).

Dickinson, Joh, Kohwi, Kohwi-Shigematsu: "A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition." dans: Cell, Vol. 70, Issue 4, pp. 631-45, (1992) (PubMed).

Détails sur SATB1

Antigène: SATB1 (SATB Homeobox 1 (SATB1))

Autre désignation: SATB1 (SATB1 Produits)

Synonymes: anticorps 2610306G12Rik, anticorps AW413156, anticorps SATB1, anticorps sb:cb955, anticorps si:ch211-195k18.2, anticorps SATB homeobox 1, anticorps special AT-rich sequence binding protein 1, anticorps SATB homeobox 1a, anticorps SATB1, anticorps Satb1, anticorps satb1, anticorps satb1a

Sujet: Packaging of fully functional eukaryotic chromosomal DNA within the nucleus requires a highly ordered organizational process. Central to this is the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, and the interaction of nucleosomes with nuclear matrix components. Such specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 (Special AT-rich sequence-Binding protein 1), expressed predominantly in the thymus, is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. These sequences tend to unwind by extensive base-unpairing in response to negative superhelical strain. SATB1 exclusively binds double stranded DNA in the minor groove with minimal base contact and it has a low affinity for supercoiled DNA. In addition, SATB1 is thought to act as a transcriptional repressor. SATB1 is a MAR binding protein that binds to the base of chromatin loops and may be essential to T cell-specific gene regulation. This antibody is routinely tested by western blot analysis.
Synonyms: Special AT-rich sequence-Binding protein-1

Poids moléculaire: 106 kDa

Pathways: Caspase Cascade in Apoptosis, Activated T Cell Proliferation