AP3D1 anticorps (AA 627-731)

N° du produit ABIN968477

Détail du produit anti-AP3D1 anticorps

Antigène: AP3D1 (Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1))

Épitope: AA 627-731

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AP3D1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Adaptin delta aa. 627-731

Clone: 18-Adaptin delta

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-AP3D1 (ABIN968477)

Blumstein, Faundez, Nakatsu, Saito, Ohno, Kelly: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, (2001) (PubMed).

Zhen, Jiang, Feng, Bright, Peden, Seymour, Novak, Elliott, Gorin, Robinson, Swank: "Abnormal expression and subcellular distribution of subunit proteins of the AP-3 adaptor complex lead to platelet storage pool deficiency in the pearl mouse." dans: Blood, Vol. 94, Issue 1, pp. 146-55, (1999) (PubMed).

Le Borgne, Alconada, Bauer, Hoflack: "The mammalian AP-3 adaptor-like complex mediates the intracellular transport of lysosomal membrane glycoproteins." dans: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29451-61, (1998) (PubMed).

Ooi, Moreira, DellAngelica, Poy, Wassarman, Bonifacino: "Altered expression of a novel adaptin leads to defective pigment granule biogenesis in the Drosophila eye color mutant garnet." dans: The EMBO journal, Vol. 16, Issue 15, pp. 4508-18, (1997) (PubMed).

Détails sur AP3D1

Antigène: AP3D1 (Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1))

Autre désignation: Adaptin delta (AP3D1 Produits)

Synonymes: anticorps wu:fj51g01, anticorps si:ch211-129c21.6, anticorps ADTD, anticorps hBLVR, anticorps AA407035, anticorps Ap3d, anticorps Bolvr, anticorps mBLVR1, anticorps mh, anticorps mocha, anticorps BLVR, anticorps adaptor-related protein complex 3, delta 1 subunit, anticorps AP-3 complex subunit delta-1, anticorps adaptor related protein complex 3 delta 1 subunit, anticorps ap3d1, anticorps EDI_172030, anticorps EHI_158840, anticorps Tsp_04773, anticorps AP3D1, anticorps Ap3d1

Sujet: Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1 through AP-4), which are associated with the vesicle coats. These coatomers contain two large adaptin proteins (gamma, alpha, delta, epsilon and beta1, beta2, beta3, beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. In the AP-3 complex, adaptin delta contains an N-terminal head domain that has 25% identity with adaptins alpha and gamma, while its C-terminal hinge and ear domains are two-fold larger and more divergent as compared to the same domains in adaptins alpha and gamma. Adaptin delta is widely expressed and its mutation results in protein storage deficiencies within lysosomal-related organelles in platelets, melansomes, and synaptic vesicles. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 160 kDa