VTI1B anticorps (AA 9-121)

N° du produit ABIN968520

Détail du produit anti-VTI1B anticorps

Antigène: VTI1B (Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))

Épitope: AA 9-121

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VTI1B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Humain, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Vti1b aa. 9-121

Clone: 7-Vti1b

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-VTI1B (ABIN968520)

Chen, Manga, Orlow: "Pink-eyed dilution protein controls the processing of tyrosinase." dans: Molecular biology of the cell, Vol. 13, Issue 6, pp. 1953-64, (2002) (PubMed).

Mallard, Tang, Galli, Tenza, Saint-Pol, Yue, Antony, Hong, Goud, Johannes: "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform." dans: The Journal of cell biology, Vol. 156, Issue 4, pp. 653-64, (2002) (PubMed).

Shorter, Beard, Seemann, Dirac-Svejstrup, Warren: "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115." dans: The Journal of cell biology, Vol. 157, Issue 1, pp. 45-62, (2002) (PubMed).

Yang, Gonzalez, Prekeris, Steegmaier, Advani, Scheller: "SNARE interactions are not selective. Implications for membrane fusion specificity." dans: The Journal of biological chemistry, Vol. 274, Issue 9, pp. 5649-53, (1999) (PubMed).

Advani, Bae, Bock, Chao, Doung, Prekeris, Yoo, Scheller: "Seven novel mammalian SNARE proteins localize to distinct membrane compartments." dans: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10317-24, (1998) (PubMed).

Détails sur VTI1B

Antigène: VTI1B (Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))

Autre désignation: Vti1b (VTI1B Produits)

Synonymes: anticorps VTI1, anticorps VTI1-LIKE, anticorps VTI1L, anticorps VTI2, anticorps v-SNARE, anticorps vti1-rp1, anticorps AU015348, anticorps GES30, anticorps MVti1b, anticorps SNARE, anticorps Vti1-rp1, anticorps zgc:63525, anticorps VTI1B, anticorps vesicle transport through interaction with t-SNAREs 1B, anticorps vesicle transport through interaction with t-SNAREs 1B pseudogene, anticorps VTI1B, anticorps Vti1b, anticorps vti1b, anticorps LOC461624

Sujet: Eukaryotic protein trafficking involves packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their contents into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs, VAMP1, VAMP2) and target proteins (t-SNAREs, syntaxin 1, SNAP-25) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. With the identification of all SNARE family members in yeast, the research focus has turned to mammalian cells. Here, sequence analysis has identified additional SNARE proteins, including VTI1a and VTI1b. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. VTI1b is a membrane bound protein whose localization overlaps with the cis/medial Golgi marker mannosidase II and the trans-Golgi marker syntaxin 6. VTI1b interacts with, and disrupts the localization of, syntaxin 5. Thus, VTI1b is thought to function in the regulation of post-Golgi vesicle trafficking.

Poids moléculaire: 27 kDa