DARPP32 anticorps (AA 70-181)

N° du produit ABIN968572

Détail du produit anti-DARPP32 anticorps

Antigène: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Épitope: AA 70-181

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DARPP32 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human DARPP-32 aa. 70-181

Clone: 15-DARPP

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-DARPP32 (ABIN968572)

Bibb, Snyder, Nishi, Yan, Meijer, Fienberg, Tsai, Kwon, Girault, Czernik, Huganir, Hemmings, Nairn, Greengard: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." dans: Nature, Vol. 402, Issue 6762, pp. 669-71, (2000) (PubMed).

Yan, Feng, Fienberg, Greengard: "D(2) dopamine receptors induce mitogen-activated protein kinase and cAMP response element-binding protein phosphorylation in neurons." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11607-12, (1999) (PubMed).

Yan, Hsieh-Wilson, Feng, Tomizawa, Allen, Fienberg, Nairn, Greengard: "Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP-32 and spinophilin." dans: Nature neuroscience, Vol. 2, Issue 1, pp. 13-7, (1999) (PubMed).

Fienberg, Hiroi, Mermelstein, Song, Snyder, Nishi, Cheramy, OCallaghan, Miller, Cole, Corbett, Haile, Cooper, Onn, Grace, Ouimet, White, Hyman, Surmeier, Girault, Nestler, Greengard: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." dans: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, (1998) (PubMed).

Kurihara, Lewis, Eisler, Greengard: "Cloning of cDNA for DARPP-32, a dopamine- and cyclic AMP-regulated neuronal phosphoprotein." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 2, pp. 508-17, (1988) (PubMed).

Détails sur DARPP32

Antigène: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Autre désignation: DARPP-32 (PPP1R1B Produits)

Synonymes: anticorps darpp32, anticorps darpp-32, anticorps Darpp-32, anticorps Darpp32, anticorps DARPP-32, anticorps DARPP32, anticorps AU040756, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 1B, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog, anticorps ppp1r1b, anticorps PPP1R1B, anticorps Ppp1r1b, anticorps ppp1r1b.S

Sujet: Dopaminergic signaling in midbrain neurons is essential to multiple brain functions and involves the activation of dopamine receptors,nsuch as D1 and D2, which regulate the phosphorylation state of DARPP-32 (dopamine and cyclic AMP-regulated phospho-protein of Mr = 32,000). D1 receptor ligation causes activation of PKA and phosphorylation of DARPP-32 at Thr-34, which converts DARPP-32 to a potent inhibitor of protein phosphatase 1 (PP1). In addition, DARPP-32 is converted to an inhibitor of PKA via phosphorylation at Thr-75 by cyclin-dependent kinase 5 (Cdk5). D2 receptor ligation inhibits PKA and activates protein phosphatase 2B/calcineurin causing dephosphorylation of DARPP-32. The major function of DARPP-32 may be to inhibit the activity of PP1, which controls the phosphorylation state of neurotransmitter receptors, ion channels, ion pumps, and transcription factors. DARPP-32 -/- mice are defective in the physiologicial and behavioral responses to dopamine. Thus, DARPP-32, a bifunctional signal transduction molecule that differentially controls a Ser/Thr kinase and a Ser/Thr phosphatase, is a critical element of dopaminergic neurotransmission and normal brain function.

Poids moléculaire: 32 kDa