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Phospholipase C beta 4 anticorps (AA 752-961)

PLCb4 Reactivité: Humain, Rat, Souris, Blow Fly WB, BI, IF Hôte: Souris Monoclonal 56-Phospholipase Cbeta4 unconjugated
N° du produit ABIN968583
  • Antigène Voir toutes Phospholipase C beta 4 (PLCb4) Anticorps
    Phospholipase C beta 4 (PLCb4)
    Épitope
    • 1
    • 1
    • 1
    AA 752-961
    Reactivité
    Humain, Rat, Souris, Blow Fly
    Hôte
    • 10
    • 1
    Souris
    Clonalité
    • 11
    Monoclonal
    Conjugué
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Phospholipase C beta 4 est non-conjugé
    Application
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), BioImaging (BI), Immunofluorescence (IF)
     Réactivité croisée
    Rat (Rattus), Souris, Mouche des fruits (Drosophila melanogaster)
    Attributs du produit
    1. Please refer to us for technical protocols.
    2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human Phospholipase Cbeta4 aa. 752-961
    Clone
    56-Phospholipase Cbeta4
    Isotype
    IgG1
  • Indications d'application
    Bioimaging:
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    Commentaires

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Kano, Hashimoto, Watanabe, Kurihara, Offermanns, Jiang, Wu, Jun, Shin, Inoue, Simon, Wu: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, (1999) (PubMed).

    Kim, Jun, Lee, Kang, Min, Kim, Ryu, Suh, Shin: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." dans: Nature, Vol. 389, Issue 6648, pp. 290-3, (1997) (PubMed).

    Alvarez, Ghalayini, Xu, Hardcastle, Bhattacharya, Rao, Pettenati, Anderson, Baehr: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." dans: Genomics, Vol. 29, Issue 1, pp. 53-61, (1996) (PubMed).

    Lee, Park, Lee, Kim, Rhee: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." dans: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, (1993) (PubMed).

  • Antigène
    Phospholipase C beta 4 (PLCb4)
    Autre désignation
    Phospholipase C beta 4 (PLCb4 Produits)
    Synonymes
    anticorps ARCND2, anticorps PI-PLC, anticorps A930039J07Rik, anticorps AI854601, anticorps C230058B11Rik, anticorps Beta4aa, anticorps RATBETA4AA, anticorps phospholipase C beta 4, anticorps phospholipase C, beta 4, anticorps PLCB4, anticorps Plcb4
    Sujet
    Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways.
    Synonyms: PLCbeta4
    Poids moléculaire
    130 kDa
    Pathways
    Signalisation WNT, Thyroid Hormone Synthesis, G-protein mediated Events
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