Nestin anticorps (AA 402-604)

N° du produit ABIN968629

Détail du produit anti-Nestin anticorps

Antigène: Nestin (NES)

Épitope: AA 402-604

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Nestin aa. 402-604 Recombinant Protein

Clone: 25-NESTIN

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Nestin (ABIN968629)

Jin, Zhu, Sun, Mao, Xie, Greenberg: "Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 18, pp. 11946-50, (2002) (PubMed).

Kernie, Erwin, Parada: "Brain remodeling due to neuronal and astrocytic proliferation after controlled cortical injury in mice." dans: Journal of neuroscience research, Vol. 66, Issue 3, pp. 317-26, (2001) (PubMed).

Steinert, Chou, Prahlad, Parry, Marekov, Wu, Jang, Goldman: "A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin." dans: The Journal of biological chemistry, Vol. 274, Issue 14, pp. 9881-90, (1999) (PubMed).

Kachinsky, Dominov, Miller: "Myogenesis and the intermediate filament protein, nestin." dans: Developmental biology, Vol. 165, Issue 1, pp. 216-28, (1994) (PubMed).

Lendahl, Zimmerman, McKay: "CNS stem cells express a new class of intermediate filament protein." dans: Cell, Vol. 60, Issue 4, pp. 585-95, (1990) (PubMed).

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin (NES Produits)

Synonymes: anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes

Sujet: The cytoskeleton consists primarily of core structural proteins that include microfilaments, microtubules, and intermediate filaments (IFs). IFs contain more than 50 distinct proteins that are organized into six different subtypes: Type I/II keratins expressed in epithelia, type III vimentin/desmin, type IV neurofilament proteins, type V nuclear lamins, and type VI nestin expressed primarily in embryonic cells. Nestin has a conserved core region (amino acids 7 to 314), which contains an alpha helical domain that is involved in coiled-coil assembly of IFs. The C-terminal region of nestin is similar to type IV IFs, since it contains highly charged amino acids, many glutamate residues, and an 11 amino acid repeat motif. Nestin is expressed in the cerebrum during embryonic development, in the cerebellum during early postnatal development, and in dermatomal cells and myoblasts during myogenesis. In vitro, nestin forms homodimers and homotetramers, but not IFs, and can co-assemble with type III vimentin and type IV internexin proteins. Thus, nestin is a core IF protein that is essential for proper cytoskeletal formation during neurogenesis and myogenesis.

Poids moléculaire: 200-260 kDa