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LIM Domain Kinase 1 anticorps (AA 232-333)

LIMK1 Reactivité: Humain, Souris, Rat WB, IF Hôte: Souris Monoclonal 42-LIMK1 unconjugated
N° du produit ABIN968654
  • Antigène Voir toutes LIM Domain Kinase 1 (LIMK1) Anticorps
    LIM Domain Kinase 1 (LIMK1)
    Épitope
    • 24
    • 15
    • 15
    • 8
    • 8
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 232-333
    Reactivité
    • 107
    • 81
    • 76
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 107
    • 15
    • 3
    Souris
    Clonalité
    • 111
    • 14
    Monoclonal
    Conjugué
    • 53
    • 11
    • 9
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp LIM Domain Kinase 1 est non-conjugé
    Application
    • 89
    • 56
    • 29
    • 26
    • 26
    • 19
    • 10
    • 8
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
     Réactivité croisée
    Rat (Rattus), Souris
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human LIMK1 aa. 232-333
    Clone
    42-LIMK1
    Isotype
    IgG1
    Top Product
    Discover our top product LIMK1 Anticorps primaire
  • Commentaires

    Related Products: ABIN968546, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." dans: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, (2000) (PubMed).

    Edwards, Gill: "Structural features of LIM kinase that control effects on the actin cytoskeleton." dans: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 11352-61, (1999) (PubMed).

    Frangiskakis, Ewart, Morris, Mervis, Bertrand, Robinson, Klein, Ensing, Everett, Green, Pröschel, Gutowski, Noble, Atkinson, Odelberg, Keating: "LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition." dans: Cell, Vol. 86, Issue 1, pp. 59-69, (1996) (PubMed).

  • Antigène
    LIM Domain Kinase 1 (LIMK1)
    Autre désignation
    LIMK1 (LIMK1 Produits)
    Synonymes
    anticorps limk1, anticorps xlimk1, anticorps GB16654, anticorps LIMK1, anticorps LIMK, anticorps LIMK-1, anticorps LIM domain kinase 1, anticorps LIM domain kinase 1a, anticorps LIM domain kinase 1 L homeolog, anticorps LIM-domain containing, protein kinase, anticorps LIMK1, anticorps limk1a, anticorps limk1, anticorps CpipJ_CPIJ000717, anticorps LOC413152, anticorps Limk1, anticorps limk1.L
    Sujet
    Two LIM motif-containing protein kinases (LIMK) have been identified, LIMK1 and LIMK2. These kinases contain two N-terminal LIM domains, a central PDZ domain, and a C-terminal Ser/Thr kinase domain. LIMK1 is highly expressed in brain, heart, and skeletal muscle, while LIMK2 exhibits the highest expression in placenta, liver, lung, kidney, and pancreas. LIMK1 is localized to the actin cytoskeleton and phosphorylates the actin binding/depolymerizing factor, cofilin. During Rho-induced neurite retraction, activation of ROCK leads to LIMK1 activation via phosphorylation at Thr508. In COS-7 cells, disruption of the second LIM domain or the PDZ domain increases LIMK1-induced aggregation of the actin cytoskeleton. In addition, a 32 kDa splice variant that contains only the N-terminus (dLIMK1) suppresses LIMK1 activity by interaction with the C- terminal kinase domain. In humans, deletion of LIMK1 has been implicated in Williams syndrome, a disorder that produces a distinct cognitive profile and vascular disease. Thus, LIMK1, and its splice variant dLIMK1, are thought to have important roles in the regulation of the actin cytoskeleton in a wide varitey of tissues.
    Poids moléculaire
    72 kDa
    Pathways
    Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
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