Beclin 1 anticorps (AA 171-291)

N° du produit ABIN968743

Détail du produit anti-Beclin 1 anticorps

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Épitope: AA 171-291

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Beclin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris, Rat (Rattus), Chien, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Beclin aa. 171-291

Clone: 20-Beclin

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Beclin 1 (ABIN968743)

Liang, Yu, Brown, Levine: "Beclin 1 contains a leucine-rich nuclear export signal that is required for its autophagy and tumor suppressor function." dans: Cancer research, Vol. 61, Issue 8, pp. 3443-9, (2001) (PubMed).

Détails sur Beclin 1

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Autre désignation: Beclin (BECN1 Produits)

Synonymes: anticorps beclin-1, anticorps atg6, anticorps vps30, anticorps beclin1, anticorps Beclin-1, anticorps becn1, anticorps zgc:73097, anticorps ATG6, anticorps VPS30, anticorps 4921513J16Rik, anticorps 5430417M23Rik, anticorps Atg6, anticorps beclin 1, anticorps beclin 1, autophagy related, anticorps beclin 1 protein, anticorps beclin-1, anticorps beclin family protein, anticorps beclin 1, autophagy related L homeolog, anticorps BECN1, anticorps becn1, anticorps atg6, anticorps CpipJ_CPIJ011087, anticorps PTRG_07825, anticorps SJAG_01876, anticorps Becn1, anticorps becn1.L

Sujet: Autophagy is the bulk degradation of cellular proteins through the autophagosomic-lysosomal pathway. This process is important for normal cell growth, and may be disrupted in tumor cells. Beclin is a Bcl-2 binding protein that is homologous to the yeast autophagy gene, apg6/vps30. The structure of beclin includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. However, inhibition of CRM1-dependent nuclear export leads to beclin localization primarily in the nucleus. Mutation of the NES domain of beclin also prevents nuclear export, as well as suppresses beclin-mediated nutrient deprivation-induced autophagy. In addition, these beclin mutants can not inhibit in vitro clonigenicity and in vivo tumorigenicity of MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since overexpression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects. Thus, beclin may be an important component of complexes involved in autophagy. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 61 kDa

Pathways: Autophagy