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LCK anticorps (pTyr505)

LCK Reactivité: Humain, Souris, Rat WB, FACS Hôte: Souris Monoclonal 4-Lck unconjugated
N° du produit ABIN968844
  • Antigène Voir toutes LCK Anticorps
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Épitope
    • 32
    • 18
    • 16
    • 11
    • 10
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr505
    Reactivité
    • 177
    • 103
    • 65
    • 9
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Humain, Souris, Rat
    Hôte
    • 152
    • 23
    • 2
    Souris
    Clonalité
    • 147
    • 31
    Monoclonal
    Conjugué
    • 100
    • 10
    • 10
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp LCK est non-conjugé
    Application
    • 132
    • 88
    • 32
    • 30
    • 25
    • 21
    • 16
    • 14
    • 13
    • 10
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
     Réactivité croisée
    Rat (Rattus), Souris
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human Lck (pY505)
    Clone
    4-Lck
    Isotype
    IgG1
    Top Product
    Discover our top product LCK Anticorps primaire
  • Commentaires

    Related Products: ABIN968537, ABIN968656, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20° C.
  • Wang, Lemay, Tsai, Veillette: "SH2 domain-mediated interaction of inhibitory protein tyrosine kinase Csk with protein tyrosine phosphatase-HSCF." dans: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1077-88, (2001) (PubMed).

    Hardwick, Sefton: "The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505." dans: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25429-32, (1997) (PubMed).

    Lee-Fruman, Collins, Burakoff: "Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation." dans: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25003-10, (1996) (PubMed).

  • Antigène
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Autre désignation
    Lck (LCK Produits)
    Synonymes
    anticorps zgc:136695, anticorps LCK, anticorps Hck-3, anticorps Lsk, anticorps Lskt, anticorps p56, anticorps p56Lck, anticorps LSK, anticorps YT16, anticorps p56lck, anticorps pp58lck, anticorps P56LCK, anticorps tkl, anticorps Lck1, anticorps Lcktkr, anticorps LCK proto-oncogene, Src family tyrosine kinase, anticorps lymphocyte protein tyrosine kinase, anticorps lck, anticorps LCK, anticorps Lck
    Sujet
    Protein tyrosine phosphorylation is an essential step in the signal transduction cascade leading to T cell antigen receptor (TCR) activation. Lck is a protein kinase and a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface MHC class II and class I molecules, respectively. Lck is regulated by both kinases and phosphatases. Autophosphorylation at Y394 leads to conformational changes in the catalytic domain, which induces kinase activity. Repression of Lck occurs via phosphorylation at Y505, located near the carboxy-terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs. Upon phosphorylation at this site, Lck associates with the SH2 domain in the amino-terminus, thus keeping the protein biologically inactive. Lck activity and regulation is critical for activation and development of T cells.
    Poids moléculaire
    56 kDa
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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