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PRKAR2B anticorps (pSer114)

PRKAR2B Reactivité: Humain, Souris, Rat WB, FACS, FM Hôte: Souris Monoclonal 47-PKA unconjugated
N° du produit ABIN968875
  • Antigène Voir toutes PRKAR2B Anticorps
    PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
    Épitope
    • 10
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    pSer114
    Reactivité
    • 61
    • 32
    • 22
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 55
    • 6
    Souris
    Clonalité
    • 54
    • 7
    Monoclonal
    Conjugué
    • 32
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PRKAR2B est non-conjugé
    Application
    • 50
    • 38
    • 12
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
     Réactivité croisée
    Souris, Rat (Rattus)
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Human PKA[RIIbeta] peptide Peptide
    Clone
    47-PKA
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product PRKAR2B Anticorps primaire
  • Indications d'application
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    Commentaires

    Related Products: ABIN968545

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20° C.
  • Budillon, Cereseto, Kondrashin, Nesterova, Merlo, Clair, Cho-Chung: "Point mutation of the autophosphorylation site or in the nuclear location signal causes protein kinase A RII beta regulatory subunit to lose its ability to revert transformed fibroblasts." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 23, pp. 10634-8, (1995) (PubMed).

    Keryer, Luo, Cavadore, Erlichman, Bornens: "Phosphorylation of the regulatory subunit of type II beta cAMP-dependent protein kinase by cyclin B/p34cdc2 kinase impairs its binding to microtubule-associated protein 2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 12, pp. 5418-22, (1993) (PubMed).

  • Antigène
    PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))
    Autre désignation
    PKA RIIbeta (PRKAR2B Produits)
    Synonymes
    anticorps MGC83177, anticorps PRKAR2B, anticorps AI451071, anticorps AW061005, anticorps PKARIIbeta, anticorps Pkarb2, anticorps RII(beta), anticorps RATDNA, anticorps PRKAR2, anticorps RII-BETA, anticorps protein kinase, cAMP-dependent, regulatory subunit type II beta S homeolog, anticorps protein kinase cAMP-dependent type II regulatory subunit beta, anticorps protein kinase, cAMP-dependent, regulatory, type II, beta, anticorps protein kinase, cAMP dependent regulatory, type II beta, anticorps protein kinase cAMP-dependent type 2 regulatory subunit beta, anticorps prkar2b.S, anticorps PRKAR2B, anticorps prkar2b, anticorps Prkar2b
    Sujet
    CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Most cells, including T lymphocytes, express both type I and type II PKAs. RIIa expression is associated with cellular transformation, while RIIbeta expression correlates with mitotic arrest and cellular differentiation. Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of the human RIIbeta subunits occurs at serine 114 (S114). In addition to their enzyme regulatory activity, the RIIalpha and RIIbeta subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins.
    The 47/PKA monoclonal antibody recognizes the phosphorylated S114 in the RIIbeta subunit of PKA. The orthologous phosphorylation site in mouse and rat PKA[RIIbeta] is S112.
    Poids moléculaire
    53 kDa
    Pathways
    Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2
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