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ENOS anticorps (pSer633)

NOS3 Reactivité: Humain WB Hôte: Souris Monoclonal 37-eNOS unconjugated
N° du produit ABIN968905
  • Antigène Voir toutes ENOS (NOS3) Anticorps
    ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
    Épitope
    • 36
    • 26
    • 22
    • 22
    • 16
    • 16
    • 15
    • 14
    • 12
    • 10
    • 10
    • 8
    • 8
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    pSer633
    Reactivité
    • 273
    • 162
    • 162
    • 14
    • 11
    • 10
    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 275
    • 36
    • 1
    Souris
    Clonalité
    • 277
    • 35
    Monoclonal
    Conjugué
    • 127
    • 27
    • 16
    • 14
    • 13
    • 12
    • 12
    • 12
    • 12
    • 12
    • 10
    • 8
    • 8
    • 8
    • 8
    • 8
    • 5
    Cet anticorp ENOS est non-conjugé
    Application
    • 261
    • 104
    • 99
    • 91
    • 79
    • 57
    • 37
    • 32
    • 25
    • 24
    • 15
    • 8
    • 7
    • 6
    • 3
    • 2
    • 1
    Western Blotting (WB)
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Human eNOS (pS633) Peptide
    Clone
    37-eNOS
    Isotype
    IgG1
    Top Product
    Discover our top product NOS3 Anticorps primaire
  • Commentaires

    Related Products: ABIN968536, ABIN967389, ABIN967886

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store undiluted at -20°C.
  • Michell, Chen Zp, Tiganis, Stapleton, Katsis, Power, Sim, Kemp: "Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." dans: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, (2001) (PubMed).

    Butt, Bernhardt, Smolenski, Kotsonis, Fröhlich, Sickmann, Meyer, Lohmann, Schmidt: "Endothelial nitric-oxide synthase (type III) is activated and becomes calcium independent upon phosphorylation by cyclic nucleotide-dependent protein kinases." dans: The Journal of biological chemistry, Vol. 275, Issue 7, pp. 5179-87, (2000) (PubMed).

    Dimmeler, Fleming, Fisslthaler, Hermann, Busse, Zeiher: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." dans: Nature, Vol. 399, Issue 6736, pp. 601-5, (1999) (PubMed).

    Gallis, Corthals, Goodlett, Ueba, Kim, Presnell, Figeys, Harrison, Berk, Aebersold, Corson: "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002." dans: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30101-8, (1999) (PubMed).

  • Antigène
    ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
    Autre désignation
    eNOS (NOS3 Produits)
    Synonymes
    anticorps ECNOS, anticorps eNOS, anticorps 2310065A03Rik, anticorps Nos-3, anticorps ecNOS, anticorps EC-NOS, anticorps NOSIII, anticorps cNOS, anticorps NOS, anticorps NOS3, anticorps eNos, anticorps ENOS, anticorps nitric oxide synthase 3, anticorps nitric oxide synthase 3, endothelial cell, anticorps NOS3, anticorps Nos3, anticorps nos3
    Sujet
    Nitirc oxide synthase (NOS), a cell type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin. eNOS has a unique N-myristylation consensus sequence that may explain its membrane localization. Various protein kinases have been implicated in regulation of eNOS activity, including AMPK, PKA, PKB/Akt, PKC, and CaM Kinase II. During VEGF stimulation, eNOS is transiently phosphorylated at Ser-1177 by PKB/Akt and dephosphorylated at Thr-495. At later time points, VEGF stimulation leads to an increase in Thr-495 phosphorylation mediated by PKC and a decrease in Ser-1177 phosphorylation. In addition, Ser-633 amd Ser-1177 are phosphorylated by PKA and PKG in vitro. Thus, eNOS activity may be regulated through complex phosphorylation events mediated by multiple kinases at various phosphorylation sites.
    Synonyms: Nitric Oxide Synthase
    Poids moléculaire
    140 kDa
    Pathways
    ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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