p63alpha anticorps

N° du produit ABIN969337

Détail du produit

Antigène: p63alpha

Reactivité: Humain, Singe, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Immunogène: Synthesized peptide of human p63alpha.

Clone: 4-00E-05

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: 4°C, -20°C for long term storage

Référence

King, Ponnamperuma, Allen, Lu, Duggal, Chen, Van Waes, Weinberg: "The p53 homologue DeltaNp63alpha interacts with the nuclear factor-kappaB pathway to modulate epithelial cell growth." dans: Cancer research, Vol. 68, Issue 13, pp. 5122-31, (2008) (PubMed).

Valenzise, Arrigo, De Luca, Privitera, Frigiola, Carando, Garelli, Silengo: "R298Q mutation of p63 gene in autosomal dominant ectodermal dysplasia associated with arrhythmogenic right ventricular cardiomyopathy." dans: European journal of medical genetics, Vol. 51, Issue 5, pp. 497-500, (2008) (PubMed).

Détail du antigène

Antigène: p63alpha

Sujet:

Description: The p63 gene is a homologue of the p53 tumor suppressor gene. Like p53, p63 contains a transactivation (TA) domain induce the transcription of target genes, a DNA binding domain, and an oligomerization domain (OD), used to form tetramers. In contrast to p53, the p63 gene encodes for at least six major isotypes. Three isotypes (TAp63α, TAp63β, and TAp63γ) contain the transactivating (TA) domain and are able to transactivate p53 report genes and induce apoptosis. In contrast, the other three isotypes (ΔNp63α, ΔNp63β, ΔNp63γ) are transcribed from an internal promoter localized within intron3, lack the TA domain, and act as dominant-negatives to suppress transactivation by both p53 and TAp63 isotypes. p63 is highly expressed in the basal cells of the epithelium significant for proper limb outgrowth and morphogenesis.4 In differentiating tissues, p63 is crucial for maintaining the stem cell identity of the basal cells, and is indispensable for correct development of the skin as well as the limb. p63-deficient mice lack all squamous epithelia and their derivatives, including hair, whiskers, teeth, as well as mammary, lacrimal, and salivary glands.Tissue specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.

Aliases: P51B, KET, p63α

Poids moléculaire: 51 kDa

ID gène: 8626

HGNC: 8626