Souris anti-Humain IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN969481

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: ELISA, Western Blotting (WB)

Attributs du produit: Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Purification: Purified

Immunogène: Human IgG was isolated from human sera and purified by chromatography.

Clone: 4D2D9G8-4A10

Isotype: IgG1

Information d'application

Indications d'application: Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C or at -20 °C for long term.

Référence

Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." dans: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet: Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Poids moléculaire: 50 kDa