IKKi/IKKe anticorps (pThr501)
Aperçu rapide pour IKKi/IKKe anticorps (pThr501) (ABIN116937)
Antigène
Voir toutes IKKi/IKKe (IKBKE) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pThr501
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Séquence
- R-S-R-L-R-pT-L-A-E-V-L
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Specificité
- This phospho specific polyclonal antibody is specific for phosphorylated pThr501 IKKE. Reactivity with non-phosphorylated IKKE is minimal.
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Purification
- Immunoaffinity Chromatography against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity.
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Immunogène
- IKKE phosphopeptide corresponding to a region of the human protein surrounding pThr501 conjugated to KLH. Genename: IKKe
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anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 495-716) antibody
IKBKE Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 686-716), (C-Term) antibodyIKBKE Reactivité: Humain WB, IHC (p) Hôte: Lapin Polyclonal RB3912 unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (C-Term) antibodyIKBKE Reactivité: Humain, Souris, Rat WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 541-640) antibodyIKBKE Reactivité: Humain ELISA, IHC (p) Hôte: Souris Monoclonal 1F7 unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) antibodyIKBKE Reactivité: Humain WB, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 544-680) antibodyIKBKE Reactivité: Humain WB, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 1-257) antibodyIKBKE Reactivité: Humain ELISA Hôte: Souris Monoclonal 6B4B5 unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) antibodyIKBKE Reactivité: Humain WB, FACS Hôte: Lapin Monoclonal 23GB2720 unconjugated Recombinant Antibody
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 686-716) antibodyIKBKE Reactivité: Humain WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (pSer172) antibodyIKBKE Reactivité: Souris, Rat WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated
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Indications d'application
- This phospho specific polyclonal antibody reacts with phosphorylated human IKKE atpT501 and shows minimal reactivity by Western blot with non-phosphorylated IKKE andminimal reactivity by ELISA against the non-phosphorylated form of the immunizingpeptide. Although not tested, this antibody is likely functional in Immunohistochemistryand Immunoprecipitation.
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Restrictions
- For Research Use only
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Concentration
- 1.15 mg/mL (by UV absorbance at 280 nm)
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Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.09 % Sodium Azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
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Autre désignation
- IKBKE / IKKE
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Sujet
- Nuclear Factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cytoplasm of the cell, which inhibit NF-κB activity. IκB proteins are phosphorylated by an IκB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-κB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IκBa.Synonyms: I kappa-B kinase epsilon, IKK-E, IKK-I, IKK-epsilon, IKKI, Inducible I kappa-B kinase, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, KIAA0151
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ID gène
- 9641
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NCBI Accession
- NP_001180250
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UniProt
- Q14164
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Pathways
- Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
Antigène
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