Enhanced Sensitivity Soluble Protein Buffer Kit

N° du produit ABIN1379844

Détail du produit

Reactivité: Souris

Application: Immunoassay (IA), Flow Cytometry (FACS)

Marque: BD CBA™

Attributs du produit: The BD™ CBA Mouse Enhanced Sensitivity Flex Set System employs particles with discrete fluorescence intensities to detect soluble analytes at very low concentrations. The working assay range for most analytes in this system is 274 to 200,000 fg/mL.
The BD CBA Mouse Enhanced Sensitivity Master Buffer Kit (Catalog Nos. 562246 and 562248) contains all of the supporting reagents necessary to perform an assay using a BD CBA Mouse Enhanced Sensitivity Flex Set.
The buffers, Enhanced Sensitivity Detection Reagent (Part B), and instrument setup reagents provided in this kit have been optimized for analysis of analytes in tissue culture supernatants and serum samples. The BD CBA Mouse Enhanced Sensitivity Master Buffer Kit provides sufficient reagents for the quantitative analysis of 100 samples and 10 instrument setup procedures

Ingrédients: Assay diluent, capture bead diluent, detection reagent diluents, wash buffer, enhanced sensitivity detection reagent, instrument setup reagents

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C

Référence

Cook, Stahl, Lowe, Chen, Morgan, Wilson, Varro, Chan, Graziano, Barney: "Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics." dans: Journal of immunological methods, Vol. 254, Issue 1-2, pp. 109-18, (2001) (PubMed).

Dotti, Savoldo, Takahashi, Goltsova, Brown, Rill, Rooney, Brenner: "Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells. A model for immunotherapy." dans: Experimental hematology, Vol. 29, Issue 8, pp. 952-61, (2001) (PubMed).

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." dans: Cytometry, Vol. 39, Issue 4, pp. 250-9, (2000) (PubMed).

Carson, Vignali: "Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay." dans: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 41-52, (1999) (PubMed).

Chen, Lowe, Wilson, Crowther, Tzeggai, Bishop, Varro: "Simultaneous Quantification of Six Human Cytokines in a Single Sample Using Microparticle-based Flow Cytometric Technology." dans: Clinical chemistry, Vol. 45, Issue 9, pp. 1693-1694, (1999) (PubMed).

Bishop, Davis: "A flow cytometric immunoassay for beta2-microglobulin in whole blood." dans: Journal of immunological methods, Vol. 210, Issue 1, pp. 79-87, (1998) (PubMed).

Oliver, Kettman, Fulton: "Multiplexed analysis of human cytokines by use of the FlowMetrix system." dans: Clinical chemistry, Vol. 44, Issue 9, pp. 2057-60, (1998) (PubMed).

McHugh: "Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes." dans: Methods in cell biology, Vol. 42 Pt B, pp. 575-95, (1995) (PubMed).