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Blotto A Pre-Mixed

BR, DB, WB 1 X
N° du produit ABIN925607
  • Application
    Blocking Reagent (BR), Dot Blot (DB), Western Blotting (WB)
    Attributs du produit
    Supplier produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to and during incubation with primary antibody.
  • Indications d'application
    Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.   Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available.  BLOTTO A is a convenient general purpose blocking agent.  BLOTTO B is recommended for blocking when phospho specific antibodies are used.  BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025).  Users should consider the addition of Tween-20, protease inhibitors and phosphatase inhibitors when required.  Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.  We recommend using 1X working solutions of BLOTTO A or BLOTTO B directly for blocking purposes and primary antibody dilution and incubation.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    1 X
    Stock
    4 °C
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