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Souris IgG2b Isotype Control

FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
N° du produit ABIN1043029
  • Antigène Tous les produits IgG2b
    IgG2b
    Hôte
    • 137
    • 59
    • 2
    Souris
    Clonalité
    • 121
    • 3
    • 2
    Monoclonal
    Application
    Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificité
    This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
    Aucune reactivité croisée
    Humain, Souris, Rat
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    KLH-coupled trinitrophenol
    Clone
    • 27
    • 17
    • 15
    • 11
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    MPC-11
    Isotype
    IgG2b kappa
  • Indications d'application
    Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Dewals, Vanderplasschen et al.: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid ..." dans: Veterinary research, Vol. 42, Issue 1, pp. 95, (2011) (PubMed).

    Liang, Zhou, Li, Wan: "Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells." dans: World journal of gastroenterology, Vol. 16, Issue 5, pp. 618-24, (2010) (PubMed).

    Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." dans: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

    Im, Tapinos, Chae, Illarionov, Besra, DeVries, Modlin, Sieling, Rambukkana, Porcelli: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, (2006) (PubMed).

    Gupta, Wang, Vinson, Dziarski: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." dans: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, (1999) (PubMed).

  • Antigène
    IgG2b
    Abstract
    IgG2b Produits
    Synonymes
    IgG2b Isotype Control, Igh-3 Isotype Control, gamma2b Isotype Control, immunoglobulin heavy constant gamma 2B Isotype Control, Ighg2b Isotype Control
    Classe de substances
    Antibody
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