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Human IgG isotype control (FITC)

IsoC, BR IgG FITC
N° du produit ABIN398849
  • Antigène Tous les produits IgG
    IgG
    Hôte
    • 144
    • 140
    • 126
    • 89
    • 88
    • 69
    • 57
    • 56
    • 47
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 15
    • 9
    • 9
    • 4
    • 2
    Human
    Conjugué
    FITC
    Application
    Isotype Control (IsoC), Blocking Reagent (BR)
    Attributs du produit
    Purified Human IgG (Whole Molecule) Control [FITC] is highly purified from normal human serum through Protein G chromatography. It is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunoblotting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction.
    Absorption Wavelength: 492 nm,
    Emission Wavelength: 518 nm,
    Fluorochrome/Protein Ratio: 4.1 moles FITC per mole of IgG
    Purification
    Protein G chromatography
    Isotype
    IgG
  • Indications d'application
    Each Investigator should determine their own optimal working dilution for specific applications.
    Commentaires

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    0.5 mg/mL
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Product is photosensitive and should be protected from light.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
  • Tomita, Fuchimoto, Mori, Kato, Uemura, Handa, Tazawa, Ohdan, Okamoto, Kuroda: "Production of anti-ABO blood group antibodies after minor ABO-incompatible bone marrow transplantation in NOD/SCID/gamma(c)(null) mice." dans: Clinical transplantation, Vol. 27, Issue 6, pp. E702-8, (2013) (PubMed).

    Lim, Kim, Aref, Kamm, Raghunath: "Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting." dans: Integrative biology : quantitative biosciences from nano to macro, Vol. 5, Issue 12, pp. 1474-84, (2013) (PubMed).

  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
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