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Souris IgG1 Isotype Control

Name: Souris IgG1 Isotype Control
Brand: antibodies-online
SKU: ABIN457406
Price: 288.82 EUR
Availability: InStock

FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) purified antibody (low endotoxin, concentration in sample 9 μg/mL).

FACS

1 image

(9 références)

N° du produit ABIN457406

Fiche de données

Antigène Tous les produits IgG1

IgG1
Hôte
221

43

27

9

4

4

2

2

1

1

1
Souris
Clonalité
177

11

6
Monoclonal

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Specificité

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.

Aucune reactivité croisée

Humain, Rat

Purification

Purified by protein-A affinity chromatography.

Pureté

> 95 % (by SDS-PAGE)

niveau d'endotoxine

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Clone
43

24

17

12

7

6

6

5

5

4

4

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
MOPC-21

Isotype

IgG1 kappa
Mouse IgG1 isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin

Mouse IgG1 isotype control (FITC)
FACS, ELISA Monoclonal 15H6 IgG1 FITC

Mouse IgG1 isotype control (PE)
FACS, ELISA Monoclonal 15H6 IgG1 PE

Mouse IgG1 isotype control
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control (SPRD)
FACS, ELISA Monoclonal 15H6 IgG1 SPRD

Mouse IgG1 isotype control (APC)
FACS, ELISA Monoclonal 15H6 IgG1 APC

Mouse IgG1 isotype control (Biotin)
FACS, ELISA Monoclonal 15H6 IgG1 Biotin

Mouse IgG1 isotype control (PE-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7

Mouse IgG1 isotype control (APC-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7

Mouse IgG1 isotype control
IsoC, ELISA, WB Monoclonal MG1L IgG1 unconjugated

Indications d'application

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4

Agent conservateur

Azide free

Conseil sur la manipulation

Do not freeze.

Stock

4 °C

Stockage commentaire

Store at 2-8°C. Do not freeze.
Piao, Na, Seo, Hong, Sohn, Kwon, Lee, Kim: "Effects of general anaesthesia with an inhalational anaesthetic agent on the expression of exosomes in rats." dans: International journal of medical sciences, Vol. 19, Issue 9, pp. 1399-1407, (2022) (PubMed).

Ni, Zhou, Song, Chen, Wang, Du, Zhang: "ELAVL1a is an immunocompetent protein that protects zebrafish embryos from bacterial infection." dans: Communications biology, Vol. 4, Issue 1, pp. 251, (2021) (PubMed).

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Rebetz, Tian, Persson, Widegren, Salford, Englund, Gisselsson, Fan: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." dans: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).

Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." dans: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." dans: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." dans: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Bryceson, March, Barber, Ljunggren, Long: "Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells." dans: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 1001-12, (2005) (PubMed).
Antigène

IgG1

Abstract

IgG1 Produits

Synonymes

IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control

Classe de substances

Antibody