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IgG Kit ELISA

Reactivité: Souris Heavy & Light Chain Colorimetric Sandwich ELISA 20-1900 ng/mL Ascitic Fluid, Cell Culture Supernatant
N° du produit ABIN1113188
  • Antigène Voir toutes IgG Kits ELISA
    IgG
    Épitope
    Heavy & Light Chain
    Reactivité
    • 9
    • 8
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    20-1900 ng/mL
    Seuil minimal de détection
    20 ng/mL
    Application
    ELISA
    Fonction
    Rapid quantification of mouse IgG in cell culture supernatant, follow-up of production process
    Marque
    FastELISA®
    Type d'échantillon
    Cell Culture Supernatant, Ascitic Fluid
    Analytical Method
    Quantitative
    Specificité
    The method enables the detection of all IgG (IgG3 quantification requires a specific standard curve).
    Réactivité croisée (Details)
    < 1% for Human IgG, < 1.5% for Cow IgG, < 2% for Goat IgG, < 5 % for Swine IgG and < 15 % for Guinea Pig and Rat IgG. The cross reaction with human serum and fetal calf serum is typically below 0.2%.
    Sensibilité
    6 ng/mL
    Attributs du produit
    The mouse immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
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  • Commentaires

    One Step ELISA 30 min total time

    Durée du test
    < 1 h
    Plaque
    Pre-coated
    Protocole
    A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
    Préparation des réactifs

    dilution

    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes IgG Kits ELISA
    IgG
    Autre désignation
    Immunoglobulin G (IgG Produits)
    Classe de substances
    Antibody
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