IgG Kit ELISA
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- Antigène Voir toutes IgG Kits ELISA
- IgG
- Épitope
- Heavy & Light Chain
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Reactivité
- Boeuf (Vache)
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 10-2000 ng/mL
- Seuil minimal de détection
- 10 ng/mL
- Application
- ELISA
- Fonction
- Rapid quantification of bovine IgG in batches of purified antibodies, selection of low IgG sera
- Marque
- FastELISA®
- Type d'échantillon
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- The method enables the detection of bovine IgG.
- Attributs du produit
- The bovine immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgG in cell culture supernatants and serums.
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- Discover our best selling IgG Kit ELISA
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- Discover our top product IgG Kit ELISA
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- Commentaires
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One Step ELISA 45 min total time
IgG contaminations of about 20 ppm - Durée du test
- < 1 h
- Plaque
- Pre-coated
- Protocole
- A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Préparation des réactifs
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dilution
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes IgG Kits ELISA
- IgG
- Autre désignation
- Immunoglobulin G (IgG Produits)
- Classe de substances
- Antibody
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