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IgG Kit ELISA

Reactivité: Boeuf (Vache) Heavy & Light Chain Colorimetric Sandwich ELISA 10-2000 ng/mL Cell Culture Supernatant, Serum
N° du produit ABIN1113190
  • Antigène Voir toutes IgG Kits ELISA
    IgG
    Épitope
    Heavy & Light Chain
    Reactivité
    • 9
    • 9
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Boeuf (Vache)
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    10-2000 ng/mL
    Seuil minimal de détection
    10 ng/mL
    Application
    ELISA
    Fonction
    Rapid quantification of bovine IgG in batches of purified antibodies, selection of low IgG sera
    Marque
    FastELISA®
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    The method enables the detection of bovine IgG.
    Attributs du produit
    The bovine immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgG in cell culture supernatants and serums.
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  • Commentaires

    One Step ELISA 45 min total time
    IgG contaminations of about 20 ppm

    Durée du test
    < 1 h
    Plaque
    Pre-coated
    Protocole
    A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
    Préparation des réactifs

    dilution

    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes IgG Kits ELISA
    IgG
    Autre désignation
    Immunoglobulin G (IgG Produits)
    Classe de substances
    Antibody
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