IgG Kit ELISA

N° du produit ABIN1113191

Détail du produit IgG Kit ELISA

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 16-2000 ng/mL

Seuil minimal de détection: 16 ng/mL

Application: ELISA

Fonction: Rapid quantification of Rat IgG in cell culture supernatant, follow-up of production process

Marque: FastELISA®

Type d'échantillon: Cell Culture Supernatant, Ascitic Fluid

Analytical Method: Quantitative

Specificité: Detection of all rat subclasses IgG.

Réactivité croisée (Details): Cross reactions with mouse IgG are < 8%.

Sensibilité: 4 ng/mL

Attributs du produit: The rat immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of rat IgG in cell culture supernatant and rat ascitic fluid.

Information d'application

Commentaires:

One Step ELISA 30 min total time

Durée du test: < 1 h

Plaque: Pre-coated

Protocole: A polyclonal antibody specific to rat IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and rat IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-rat IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of rat IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Préparation des réactifs:

dilution

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Autre désignation: Immunoglobulin G (IgG Produits)

Classe de substances: Antibody