CXCL6 Kit ELISA

N° du produit ABIN1672728

Détail du produit CXCL6 Kit ELISA

Antigène: CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))

Épitope: AA 40-114

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL6/GCP2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: V40-N114

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: V40-N114

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Plaque: Pre-coated

Protocole: human CXCL6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL6 has been precoated onto 96-well plates. Standards(E.coli, V40-N114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL6 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL6 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 223, Standard deviation: 13.6, CV(%): 6.1
• Sample 2: n=16, Mean(pg/ml): 519, Standard deviation: 33.2, CV(%): 6.4
• Sample 3: n=16, Mean(pg/ml): 1089, Standard deviation: 64.3, CV(%): 5.9,
• Sample 1: n=24, Mean(pg/ml): 245, Standard deviation: 18.1, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 542, Standard deviation: 41.2, CV(%): 7.6
• Sample 3: n=24, Mean(pg/ml): 1054, Standard deviation: 71.7, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))

Autre désignation: CXCL6 (CXCL6 Produits)

Synonymes: CKA-3 Kit ELISA, GCP-2 Kit ELISA, GCP2 Kit ELISA, SCYB6 Kit ELISA, Cxcl6 Kit ELISA, CXCL5 Kit ELISA, C-X-C motif chemokine ligand 6 Kit ELISA, chemokine (C-X-C motif) ligand 5 Kit ELISA, CXCL6 Kit ELISA, Cxcl6 Kit ELISA, CXCL5 Kit ELISA

Sujet:

Protein Function: Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram- negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7). .

Background: Chemokine(C-X-C motif) ligand 6(CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2(GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2. The gene for CXCL6 is located on human chromosome 4 in a cluster with other CXC chemokine genes.

Synonyms: C-X-C motif chemokine 6,Chemokine alpha 3,CKA-3,Granulocyte chemotactic protein 2,GCP-2,Small-inducible cytokine B6,Small-inducible cytokine B6, N-processed variant 1,Small-inducible cytokine B6, N-processed variant 2,Small-inducible cytokine B6, N-processed variant 3,CXCL6,GCP2, SCYB6,

Full Gene Name: C-X-C motif chemokine 6

Cellular Localisation: Secreted.

ID gène: 6372

UniProt: P80162