CCL13 Kit ELISA

N° du produit ABIN1706066

Détail du produit CCL13 Kit ELISA

Antigène: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Épitope: AA 24-98

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8-500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL13/MCP4

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: Q24-T98

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: Q24-T98

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Tissue Specificity: Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

Plaque: Pre-coated

Protocole: human CCL13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL13 has been precoated onto 96-well plates. Standards(E.coli, Q24-T98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL13 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CCL13 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 50, Standard deviation: 2.15, CV(%): 4.3
• Sample 2: n=16, Mean(pg/ml): 191, Standard deviation: 8.98, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 325, Standard deviation: 20.15, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 54, Standard deviation: 3.13, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 223, Standard deviation: 14.05, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 356, Standard deviation: 27.77, CV(%): 7.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CCL13 Kit ELISA (ABIN1706066)

Garcia-Zepeda, Combadiere, Rothenberg, Sarafi, Lavigne, Hamid, Murphy, Luster et al.: "Human monocyte chemoattractant protein (MCP)-4 is a novel CC chemokine with activities on monocytes, eosinophils, and basophils induced in allergic and nonallergic inflammation that signals through ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5613-26, (1997) (PubMed).

Détail du antigène

Antigène: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Autre désignation: CCL13 (CCL13 Produits)

Synonymes: CKb10 Kit ELISA, MCP-4 Kit ELISA, NCC-1 Kit ELISA, NCC1 Kit ELISA, SCYA13 Kit ELISA, SCYL1 Kit ELISA, C-C motif chemokine ligand 13 Kit ELISA, chemokine (C-C motif) ligand 13 Kit ELISA, CCL13 Kit ELISA

Sujet:

Protein Function: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Background: Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Synonyms: C-C motif chemokine 13,CK-beta-10,Monocyte chemoattractant protein 4,Monocyte chemotactic protein 4,MCP-4,NCC-1,Small-inducible cytokine A13,C-C motif chemokine 13, long chain,C-C motif chemokine 13, medium chain,C-C motif chemokine 13, short chain,CCL13,MCP4, NCC1, SCYA13,

Full Gene Name: C-C motif chemokine 13

Cellular Localisation: Secreted.

ID gène: 6357

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Phosphorylation & l'infection par le SRAS-CoV-2