SensoLyte® 520 TACE (a-Secretase) Activity Assay Kit

N° du produit ABIN1882558

Détail du produit

Antigène: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivité: Humain

Méthode de détection: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marque: SensoLyte®

Attributs du produit: SensoLyte® 520 TACE Activity Assay Kit uses a 5-FAM (fluorophore) and QXL™ 520 (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The kit can be used to detect the activity of TACE or screen for TACE inhibitors. The assays are performed in a convenient 96-well format.

Information d'application

Commentaires:

FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect Components A and B from light and from moisture.

Stock: -20 °C

Stockage commentaire: Store all kit components at -20 °C. Components C and E can be stored at room temperature for convenience.

Référence

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Détail du antigène

Antigène: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Autre désignation: TACE (ADAM17 Produits)

Sujet: TACE (tumor necrosis factor-alpha converting enzyme), alpha-secretase, or ADAM17 is a member of the ADAM family of proteases that contain both disintegrin and metalloprotease (catalytic) domains. This enzyme is involved in sperm-egg binding and fusion, muscle cell fusion, neurogenesis, modulation of Notch receptor and ligand processing, and processing of the pro-inflammatory cytokine, TNFalpha. TACE is currently being explored as a target for anti-inflammatory drugs.