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NFkB Kit ELISA

NFkB Reactivité: Humain Colorimetric DNA-Binding ELISA Cell Extracts, Tissue Samples
N° du produit ABIN2866266
  • Antigène Voir toutes NFkB Kits ELISA
    NFkB (Nuclear Factor kappa B (NFkB))
    Reactivité
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    DNA-Binding ELISA
    Application
    ELISA
    Fonction
    DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
    Marque
    TransAM®
    Type d'échantillon
    Cell Extracts, Tissue Samples
    Analytical Method
    Quantitative
    Specificité
    The TransAM Flexi NFκB Family Kit is tested for sensitivity and specificity in detecting NFκB activation.
    Attributs du produit
    Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
    TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.
    Ingrédients
    One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    Nuclear extracts are prepared from Raji cells and 5 μg/well are assayed using 1:1000 dilutions of the NFκB p65, p50, p52, RelB and c-Rel antibodies found in the TransAM NFκB Family Kit. The relative activation of each family member may vary depending on the cell type tested and the manner in which it was stimulated. TransAM assays are performed in the absence or presence of 40 pmol of oligonucleotide that contains either a wild-type or mutated NFκB consensus binding site (Figure 1). The wild-type oligonucleotide prevents NFκB binding to the biotinylated probe. Conversely, the mutated oligonucleotide has a limited effect on NFκB binding. For the experiment shown in Figure 1, development times for the assay were: NFκB p65: 8 minutes NFκB p50: 2 minutes p52: 5 minutes Rel B: 5 minutes c-Rel: 3 minutes Individual optimization of development times may be required.

    Durée du test
    5 h
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C/-20 °C/-80 °C
    Stockage commentaire
    Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, the c-Rel, p50 and p65 antibodies, DTT, Herring sperm DNA and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.
    Date de péremption
    6 months
  • Antigène Voir toutes NFkB Kits ELISA
    NFkB (Nuclear Factor kappa B (NFkB))
    Autre désignation
    Nfkb (NFkB Produits)
    Synonymes
    EBP-1 Kit ELISA, KBF1 Kit ELISA, NF-kB1 Kit ELISA, NF-kappa-B Kit ELISA, NF-kappaB Kit ELISA, NFKB-p105 Kit ELISA, NFKB-p50 Kit ELISA, NFkappaB Kit ELISA, p105 Kit ELISA, p50 Kit ELISA, NF-kB Kit ELISA, NF-KB1 Kit ELISA, NF-kappaB1 Kit ELISA, p50/p105 Kit ELISA, nuclear factor kappa B subunit 1 Kit ELISA, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Kit ELISA, NFKB1 Kit ELISA, Nfkb1 Kit ELISA
    Pathways
    Ubiquitin Proteasome Pathway, S100 Proteins
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