SOAT1 Kit ELISA

N° du produit ABIN2866293

Détail du produit SOAT1 Kit ELISA

Antigène: SOAT1 (Sterol O-Acyltransferase 1 (SOAT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Marque: TransAM®

Type d'échantillon: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificité: The TransAM STAT Family Kit is tested for sensitivity and specificity in detecting STAT activation.

Attributs du produit: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Ingrédients: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Using 1:1000 dilutions of STAT1, STAT5A and STAT5B antibodies and a 1:500 dilution of STAT3 antibody provided in the kit are tested using 5 μg/well of nuclear extract prepared from a stimulated cell line: STAT1a is tested with U-937 (TPA + IFNg), STAT3 with HepG2 (IL-6, 100 ng/ml), and STAT5A and STAT5B with Nb2 (Prolactin). The relative activation of each family member may vary depending on the cell type tested and the manner in which it was stimulated. TransAM assays are performed in the absence or presence of 20 pmol of competitor oligonucleotide that contains either a wild-type or mutated STAT consensus binding site (Figure 1). The wild-type oligonucleotide prevents STAT binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has a limited effect on STAT binding.

Durée du test: 5 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Store the cell extracts at -80°C. All other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, the antibodies, DTT and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extracts at -80°C. This product is guaranteed for 6 months from date of receipt.

Date de péremption: 6 months

Détail du antigène

Antigène: SOAT1 (Sterol O-Acyltransferase 1 (SOAT1))

Autre désignation: Stat (SOAT1 Produits)

Synonymes: si:dkey-21p1.4 Kit ELISA, ACACT Kit ELISA, ACAT Kit ELISA, ACAT-1 Kit ELISA, ACAT1 Kit ELISA, SOAT Kit ELISA, STAT Kit ELISA, 8430426K15Rik Kit ELISA, AW550831 Kit ELISA, Acact Kit ELISA, ald Kit ELISA, hid Kit ELISA, Acat-1 Kit ELISA, Acat Kit ELISA, sterol O-acyltransferase 1 S homeolog Kit ELISA, sterol O-acyltransferase 1 Kit ELISA, sterol O-acyltransferase Kit ELISA, soat1.S Kit ELISA, SOAT1 Kit ELISA, soat1 Kit ELISA, CpipJ_CPIJ019799 Kit ELISA, PTRG_01998 Kit ELISA, PTRG_00080 Kit ELISA, Smp_134390 Kit ELISA, CNF01130 Kit ELISA, CC1G_00886 Kit ELISA, Soat1 Kit ELISA