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MMP8 Kit ELISA

MMP8 Reactivité: Humain AA 21-467 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum
N° du produit ABIN411332
  • Antigène Voir toutes MMP8 Kits ELISA
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    Épitope
    AA 21-467
    Reactivité
    • 7
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-8
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Saliva
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: F21-G467
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: F21-G467
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the peptidase M10A family.

    Tissue Specificity: Neutrophils.

    Plaque
    Pre-coated
    Protocole
    human MMP-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-8 has been precoated onto 96-well plates. Standards(NSO, F21-G467) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-8 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-8 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 1275, Standard deviation: 56.1, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 3563, Standard deviation: 185.3, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 395.7, CV(%): 5.8,
    • Sample 1: n=24, Mean(pg/ml): 1370, Standard deviation: 69.87, CV(%): 5.1
    • Sample 2: n=24, Mean(pg/ml): 3728, Standard deviation: 242.32, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 7142, Standard deviation: 521.4, CV(%): 7.3
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Kumar, Jain, Chauhan, Bharate, Koli, Kumar: "Influence of different forms and materials (zirconia or titanium) of abutments in periimplant soft-tissue healing using matrix metalloproteinase-8: A randomized pilot study." dans: The Journal of prosthetic dentistry, (2017) (PubMed).

    Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." dans: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

    Akbari, Prabhuji, Karthikeyan, Raghunatha, Narayanan: "Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test." dans: Journal of Indian Society of Periodontology, Vol. 19, Issue 5, pp. 525-30, (2015) (PubMed).

  • Antigène Voir toutes MMP8 Kits ELISA
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    Autre désignation
    MMP8 (MMP8 Produits)
    Synonymes
    MMP8 Kit ELISA, BB138268 Kit ELISA, CLG1 Kit ELISA, HNC Kit ELISA, MMP-8 Kit ELISA, PMNL-CL Kit ELISA, matrix metallopeptidase 8 Kit ELISA, neutrophil collagenase Kit ELISA, MMP8 Kit ELISA, Mmp8 Kit ELISA, LOC100339790 Kit ELISA
    Sujet

    Protein Function: Can degrade fibrillar type I, II, and III collagens.

    Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

    Synonyms: Neutrophil collagenase,3.4.24.34,Matrix metalloproteinase-8,MMP-8,PMNL collagenase,PMNL-CL,MMP8,CLG1,

    Full Gene Name: Neutrophil collagenase

    Cellular Localisation: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.
    ID gène
    4317
    UniProt
    P22894
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