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VCAM1 Kit ELISA

VCAM1 Reactivité: Humain AA 25-698 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN411367
  • Antigène Voir toutes VCAM1 Kits ELISA
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    Épitope
    AA 25-698
    Reactivité
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human VCAM-1
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO,F25 - E698
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: F25 - E698
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

    Tissue Specificity: Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

    Plaque
    Pre-coated
    Protocole
    human VCAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VCAM-1 has been precoated onto 96-well plates. Standards (NSO,F25 - E698) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VCAM-1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human VCAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human VCAM-1 standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 560, Standard deviation: 24.08, CV(%): 4.3
    • Sample 2: n=16, Mean(pg/ml): 3210, Standard deviation: 160.5, CV(%): 5
    • Sample 3: n=16, Mean(pg/ml): 6560, Standard deviation: 229.6, CV(%): 3.5,
    • Sample 1: n=24, Mean(pg/ml): 450, Standard deviation: 26.1, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 2860, Standard deviation: 185.9, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 5890, Standard deviation: 282.72, CV(%): 4.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Dehghan, Sabri, Javanmard, Ahmadi, Mansourian: "Neurally mediated syncope: Is it really an endothelial dysfunction?" dans: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).

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  • Antigène Voir toutes VCAM1 Kits ELISA
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    Autre désignation
    VCAM1 (VCAM1 Produits)
    Synonymes
    CD106 Kit ELISA, INCAM-100 Kit ELISA, Vcam-1 Kit ELISA, VCAM1B Kit ELISA, fi76d06 Kit ELISA, wu:fi76d06 Kit ELISA, zgc:158875 Kit ELISA, VCAM1 Kit ELISA, vascular cell adhesion molecule 1 Kit ELISA, vascular cell adhesion molecule 1b Kit ELISA, VCAM1 Kit ELISA, Vcam1 Kit ELISA, vcam1b Kit ELISA, VCAM-1 Kit ELISA
    Sujet

    Protein Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

    Background: Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

    Synonyms: Vascular cell adhesion protein 1,V-CAM 1,VCAM-1,INCAM-100,CD106,VCAM1,L1CAM,

    Full Gene Name: Vascular cell adhesion protein 1

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    7412
    UniProt
    P19320
    Pathways
    Carbohydrate Homeostasis
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