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MMP2 Kit ELISA

MMP2 Reactivité: Rat AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
N° du produit ABIN411386
  • Antigène Voir toutes MMP2 Kits ELISA
    MMP2 (Matrix Metalloproteinase 2 (MMP2))
    Épitope
    AA 30-662
    Reactivité
    • 9
    • 8
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    625-40000 pg/mL
    Seuil minimal de détection
    625 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat MMP-2
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: A30-C662
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: A30-C662
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Belongs to the peptidase M10A family.

    Plaque
    Pre-coated
    Protocole
    rat MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-2 has been precoated onto 96-well plates. Standards(NSO, A30-C662) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MMP-2 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 40000pg/mL, 20,000pg/mL,10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL rat MMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MMP-2 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 1358, Standard deviation: 59.8, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 3628, Standard deviation: 206.8, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 6327, Standard deviation: 399, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 1526, Standard deviation: 82.4, CV(%): 5.4
    • Sample 2: n=24, Mean(pg/ml): 3874, Standard deviation: 240.2, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 6556, Standard deviation: 465.5, CV(%): 7.1
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • You, Zu, Liu, Kong, Song, Wei, Zhou, Wang, Yan: "Synovial fibroblast-targeting liposomes encapsulating an NF-κB-blocking peptide ameliorates zymosan-induced synovial inflammation." dans: Journal of cellular and molecular medicine, Vol. 22, Issue 4, pp. 2449-2457, (2018) (PubMed).

    Zhang, Shi, Zou, Chen, Tang, Ye, Liu: "KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin." dans: Experimental and therapeutic medicine, Vol. 14, Issue 1, pp. 135-140, (2017) (PubMed).

    Zhang, Li, Liu, Wang, Chen, Xing, Zhu: "STAT3-mediated MMP-2 expression is required for 15-HETE-induced vascular adventitial fibroblast migration." dans: The Journal of steroid biochemistry and molecular biology, Vol. 149, pp. 106-17, (2015) (PubMed).

    Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." dans: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).

    Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." dans: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

    Liu, Zou, Zhou, Gong, Wang, Cai, Tan, Fang: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." dans: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).

    Qin, Liu, Li, Liu, Li, Gao, Shao, Zhen: "MMP-2/9-oriented combinations enhance antitumor efficacy of EGFR/HER2-targeting fusion proteins and gemcitabine." dans: Oncology reports, Vol. 32, Issue 1, pp. 121-30, (2014) (PubMed).

    Yuan, Yang, Zhou, Gao, Qiu, Fang, Ding, Xiao et al.: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." dans: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).

    Mazani, Fard, Baghi, Nemati, Mogadam: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." dans: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).

    Zhang, Sun, Zhang, Ge, Liu, Zhao, Lu, Fan: "Norcantharidin inhibits tumor growth and vasculogenic mimicry of human gallbladder carcinomas by suppression of the PI3-K/MMPs/Ln-5?2 signaling pathway." dans: BMC cancer, Vol. 14, pp. 193, (2014) (PubMed).

    Rizk, El-Maraghy, Nassar: "A novel role for SIRT-1 in L-arginine protection against STZ induced myocardial fibrosis in rats." dans: PLoS ONE, Vol. 9, Issue 12, pp. e114560, (2014) (PubMed).

    Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." dans: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

    Tang, Tang, Fang, Liang, Zhang: "Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells." dans: World journal of gastroenterology, Vol. 19, Issue 10, pp. 1582-92, (2013) (PubMed).

    Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." dans: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

    Pan, Ren, Ma, Liu, Yu, Ji, Pan, Li, Yang, Lv, Shen, Chen, Zhang, Willard, He, Zheng: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." dans: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).

    Yu, Zhu, Mi, Chen, Pan, Wei: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." dans: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).

    Zhang, Chen, Qi, Wang, Xiao, Zhu: "Inhibition of calcium-calmodulin-dependent kinase II suppresses cardiac fibroblast proliferation and extracellular matrix secretion." dans: Journal of cardiovascular pharmacology, Vol. 55, Issue 1, pp. 96-105, (2010) (PubMed).

  • Antigène Voir toutes MMP2 Kits ELISA
    MMP2 (Matrix Metalloproteinase 2 (MMP2))
    Autre désignation
    MMP2 (MMP2 Produits)
    Synonymes
    2-MMP Kit ELISA, CG1794 Kit ELISA, DM2-MMP Kit ELISA, Dm2-MMP Kit ELISA, Dmel\\CG1794 Kit ELISA, MMP2 Kit ELISA, anon-WO0118547.84 Kit ELISA, dm-2MMP Kit ELISA, dmmp2 Kit ELISA, l(2)02353 Kit ELISA, mmp2 Kit ELISA, wu:fa99h12 Kit ELISA, wu:fk89d01 Kit ELISA, fgmmp-2 Kit ELISA, Mmp-2 Kit ELISA, LOC100135793 Kit ELISA, Clg4a Kit ELISA, GelA Kit ELISA, MMP-2 Kit ELISA, CLG4 Kit ELISA, CLG4A Kit ELISA, MMP-II Kit ELISA, MONA Kit ELISA, TBE-1 Kit ELISA, matrix metallopeptidase 2 Kit ELISA, Matrix metalloproteinase 2 Kit ELISA, matrix metalloproteinase 2 Kit ELISA, matrix metalloproteinase-2 Kit ELISA, MMP2 Kit ELISA, Mmp2 Kit ELISA, mmp2 Kit ELISA, LOC657982 Kit ELISA, LOC100135793 Kit ELISA
    Sujet

    Protein Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity). .

    Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

    Synonyms: 72 kDa type IV collagenase,3.4.24.24,72 kDa gelatinase,Gelatinase A,Matrix metalloproteinase-2,MMP-2,PEX,Mmp2,

    Full Gene Name: 72 kDa type IV collagenase

    Cellular Localisation: Secreted, extracellular space, extracellular matrix . Membrane . Nucleus . Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity)..
    ID gène
    81686
    UniProt
    P33436
    Pathways
    Activation of Innate immune Response
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