TSH Kit ELISA

N° du produit ABIN415519

Détail du produit TSH Kit ELISA

Antigène: TSH (Thyroid Stimulating Hormone (TSH))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 6.4 pg/mL - 4000 pg/mL

Seuil minimal de détection: 6.4 pg/mL

Application: ELISA

Fonction: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of TSH in mouse serum, plasma.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH).
No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.

Sensibilité: 2.2 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 50μL standard or sample to each well.
   Then add 50μL prepared Detection Reagent A immediately.
   Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 3 times,
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
5. Aspirate and wash 5 times,
6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 0.4 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 4,000pg/mL. Please prepare 5 tubes containing 0.4 mL Standard Diluent and produce a quintuple dilution series according to the picture shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 4,000pg/mL, 800pg/mL, 160pg/mL, 32pg/mL, 6.4pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. The standard for this kit is liquid. Due to its small volume, maybe invisible to the eye.
2. Making serial dilution in the wells directly is not permitted.
3. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
4. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
5. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
6. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
7. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
8. Contaminated water or container for reagent preparation will influence the detection result.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Independent Validation (ELISA)

Validation #100110 (ELISA)

by: Zentrum fuer Augenheilkunde, Unversitätsklinikum Essen, Universität Duisburg-Essen

No.: #100110

Date: 14.06.2017

Antigène: mTSH

Numéro du lot: L170428173

Application validée: ELISA

Contrôle positif:

euthyroid mouse serum

low (50pg/ml), medium (250pg/ml), and high (2500pg/ml) mTSH concentration reference standards (Cloud Clone) undiluted or diluted 1:2 in Standard Diluent

Contrôle négative:

Blank samples Standard Diluent only

Conclusion:

Passed, the murine TSH ELISA kit ABIN415519 specifically detects the antigen in euthyroid mouse serum.

Validation Images

A. Standard curve for ABIN415519, generated from one measurement of the absorbance of the kit standard diluted as described in the protocol section at 450nm. B. Plotted standard curve. C. Measurement of absorbance values at 450nm and calculated concentration of the tested samples.

Protocole

Full Protocol:

• Bring all reagents and samples to room temperature before use.
• Prepare the standards as described in the kit manual. It is very important to allow the stock to sit for 10min and the prepared standards for 15min at RT.
• Pipette 50µl of the diluted kits standard, blank, and the sample to each well diluted as indicated above.
• Add immediately 50µl Detection Reagent A diluted 1:100in Assay Diluent A to each well.
• Seal plate with an adhesive strip and mix the plate gently and let it incubate for 1h at 37°C.
• Remove the solution and wash the wells 4x for 1min with 350µl Wash Solution diluted to 1x in distilled H₂O.
• After the last washing step, remove the remaining liquid by inverting and blotting the plate against absorbent paper.
• Add 100µl Detection Reagent B diluted 1:100 in Assay Diluent B to each well.
• Seal plate with an adhesive strip and let the plate incubate for 30min at 37°C.
• Remove the solution and wash 5x for 1min 350µl Wash Solution diluted to 1x in distilled H₂O.
• Add 90µl TMB Substrate.
• Seal plate with an adhesive strip and incubate the plate for 15min at 37°C.
• Add 50µl Stop Solution, mix the plate gently and measure immediately the absorbance at 450nm.

Notes:

• The Assay is very sensitive. Therefore it is very important to check all reagents before use. Do not use any reagents that appear cloudy.
• In addition to that it is necessary to store the kit under the recommended conditions. If the entire kit is used at once, it is necessary aliquot the reagents and take out only the strips for the present assay.
• All diluted reagents have to be mixed very gently.

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour TSH Kit ELISA (ABIN415519)

Paulazo, Klecha, Sterle, Valli, Torti, Cayrol, Barreiro Arcos, Cremaschi: "Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity." dans: Endocrine, Vol. 66, Issue 2, pp. 266-277, (2020) (PubMed).

Wu, Cai, Xia, Liu, Yang, Wang, Wang, Yu, Yin, Wang, Zhu: "Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice." dans: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 94, (2020) (PubMed).

Yang, Xiang, Zhang, Shan, Li, Teng: "The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice." dans: Clinical immunology (Orlando, Fla.), Vol. 212, pp. 108350, (2020) (PubMed).

Yoo, Cha, Kim, Jiang, Cooke, Adebesin, Wolfe, Riddle, Aja, Blackshaw: "Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice." dans: Glia, Vol. 68, Issue 10, pp. 1987-2000, (2020) (PubMed).

Lu, Qin, Xiang, Sun, Feng, Zhang, Ding, Li, Shan, Teng: "Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy." dans: International immunopharmacology, Vol. 85, pp. 106563, (2020) (PubMed).

Wang, Sun, Yang, Xu, Cai, Liu, Xia, Zhu, Wang: "Hashimoto's Thyroiditis Induces Hippocampus-Dependent Cognitive Alterations by Impairing Astrocytes in Euthyroid Mice." dans: Thyroid : official journal of the American Thyroid Association, (2020) (PubMed).

Yang, Chin, Hsieh, Lai, Ke, Crawford, Lee, Fu, Mousa, Grasso, Liu, Chang, Tang, Lin, Davis: "Novel leptin OB3 peptide-induced signaling and progression in thyroid cancers: Comparison with leptin." dans: Oncotarget, Vol. 7, Issue 19, pp. 27641-54, (2018) (PubMed).

Cai, Wang, Chen, Li, Fan, Wu, Ge, Hu, Wang, Zhu: "Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 299, (2018) (PubMed).

Cao, Hua, Xiong, Zhu, Zhang, Chen: "Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice." dans: Frontiers in molecular neuroscience, Vol. 11, pp. 6, (2018) (PubMed).

Tang, Yin, Zhang, Chen, Jin, Liu: "Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population." dans: Environmental pollution (Barking, Essex : 1987), Vol. 237, pp. 581-591, (2018) (PubMed).

Qatato, Szumska, Skripnik, Rijntjes, Köhrle, Brix: "Canonical TSH Regulation of Cathepsin-Mediated Thyroglobulin Processing in the Thyroid Gland of Male Mice Requires Taar1 Expression." dans: Frontiers in pharmacology, Vol. 9, pp. 221, (2018) (PubMed).

Cao, Hua, Wang, Chen: "Exposure of pregnant mice to triclosan impairs placental development and nutrient transport." dans: Scientific reports, Vol. 7, pp. 44803, (2017) (PubMed).

Herriges, Tischfield, Cui, Morley, Han, Babu, Li, Lu, Cendan, Garcia, Anderson, Morrisey: "The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis." dans: Genes & development, Vol. 31, Issue 9, pp. 889-903, (2017) (PubMed).

Le Stunff, Tilotta, Sadoine, Le Denmat, Briet, Motte, Clauser, Bougnères, Chaussain, Silve: "Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis." dans: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 32, Issue 2, pp. 333-346, (2016) (PubMed).

Feng, Cao, Zhao, Wang, Hua, Chen, Chen: "Exposure of Pregnant Mice to Perfluorobutanesulfonate Causes Hypothyroxinemia and Developmental Abnormalities in Female Offspring." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 155, Issue 2, pp. 409-419, (2016) (PubMed).

OHara, Curley, Tedim Ferreira, Cruickshanks, Milne, Smith: "Pituitary androgen receptor signalling regulates prolactin but not gonadotrophins in the male mouse." dans: PLoS ONE, Vol. 10, Issue 3, pp. e0121657, (2015) (PubMed).

Zhang, Song, Feng, Zhou, Lu, Gao, Yu, Jiang, Zhao: "Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver." dans: Journal of lipid research, Vol. 56, Issue 5, pp. 963-71, (2015) (PubMed).

Abdulrahman, Boon, Sips, Guigas, Rensen, Smit, Hovens: "Impact of Metformin and compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function." dans: Thyroid : official journal of the American Thyroid Association, Vol. 24, Issue 1, pp. 78-87, (2014) (PubMed).

Sterle, Valli, Cayrol, Paulazo, Martinel Lamas, Diaz Flaqué, Klecha, Colombo, Medina, Cremaschi, Barreiro Arcos: "Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis." dans: The Journal of endocrinology, Vol. 222, Issue 2, pp. 243-55, (2014) (PubMed).

Ichijo, Furuya, Shimura, Hayashi, Takahashi, Ohta, Kobayashi, Kitamura: "Activation of the RhoB signaling pathway by thyroid hormone receptor ? in thyroid cancer cells." dans: PLoS ONE, Vol. 9, Issue 12, pp. e116252, (2014) (PubMed).

Détail du antigène

Antigène: TSH (Thyroid Stimulating Hormone (TSH))

Autre désignation: TSH (TSH Produits)

Synonymes: CG7665 Kit ELISA, DLGR-1 Kit ELISA, DLGR1 Kit ELISA, Dmel\CG7665 Kit ELISA, FSH Kit ELISA, FSH-TSH Kit ELISA, Fsh Kit ELISA, Fsh/CG7665 Kit ELISA, LGR1 Kit ELISA, LH/CG receptor Kit ELISA, RH44949p Kit ELISA, TSH Kit ELISA, dLGR1 Kit ELISA, Leucine-rich repeat-containing G protein-coupled receptor 1 Kit ELISA, Lgr1 Kit ELISA

Classe de substances: Hormone