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Exosome Purification and Analysis Kit (Cell culture)

FACS Reactivité: Humain Fluorometric Cell Culture Cells
N° du produit ABIN5633279
  • Reactivité
    Humain
    Méthode de détection
    Fluorometric
    Conjugué
    PE
    Application
    Flow Cytometry (FACS)
    Fonction
    Exosome purification and FACS analysis kit of exosomes derived from cell culture
    Marque
    ExoStep™
    Type d'échantillon
    Cell Culture Cells
    Attributs du produit
    The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
    Ingrédients
    CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X
    Matériel non inclus
    • Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer
  • tubes
  • ). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume
  • Indications d'application
    Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes
    Commentaires

    Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol

    Volume d'échantillon
    50 μL
    Durée du test
    4 h
    Préparation des réactifs

    Reagents ready to use.

    Restrictions
    For Research Use only
  • Buffer
    The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store in the dark at 2-8 °C
  • Sujet
    Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
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