Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

SERPINA1 Kit ELISA

SERPINA1 Reactivité: Souris Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5651612
  • Antigène Voir toutes SERPINA1 Kits ELISA
    SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
    Reactivité
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 ng/mL - 2000 ng/mL
    Seuil minimal de détection
    31.25 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT). No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.
    Sensibilité
    12.8 ng/mL
    Featured
    Discover our best selling SERPINA1 Kit ELISA
    Top Product
    Discover our top product SERPINA1 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes SERPINA1 Kits ELISA
    SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
    Autre désignation
    Alpha-1-Antitrypsin (SERPINA1 Produits)
    Synonymes
    AACT Kit ELISA, ACT Kit ELISA, GIG25 Kit ELISA, A1AT Kit ELISA, PI Kit ELISA, alpha-1-PI Kit ELISA, A1A Kit ELISA, AAT Kit ELISA, PI1 Kit ELISA, PRO2275 Kit ELISA, alpha1AT Kit ELISA, Pi Kit ELISA, Spi1 Kit ELISA, MGC64429 Kit ELISA, serpina1d Kit ELISA, Aps Kit ELISA, SERPINA3-5 Kit ELISA, serpin family A member 3 Kit ELISA, alpha-1-antiproteinase F precursor Kit ELISA, serpin family A member 1 Kit ELISA, alpha-1-antitrypsin Kit ELISA, calumenin Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Kit ELISA, SH2B adaptor protein 2 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog Kit ELISA, SERPINA3 Kit ELISA, LOC100008973 Kit ELISA, SERPINA1 Kit ELISA, LOC100328621 Kit ELISA, CALU Kit ELISA, Serpina1 Kit ELISA, serpina1.S Kit ELISA, serpina1 Kit ELISA, SPIA5 Kit ELISA, Sh2b2 Kit ELISA, Calu Kit ELISA, serpina1.L Kit ELISA
    Sujet

    Gene Name: Alpha-1-Antitrypsin

    Gene Aliases: SERPINA1, SPAAT, A1-AT, PI, Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1, Alpha-1 protease inhibitor, Short peptide from AAT, Serpin A1

    ID gène
    20700
    UniProt
    P07758
Vous êtes ici:
Support technique